干旱胁迫对花生PnAG_1基因的表达及生理指标的影响  

Effects of Drought Stress on Peanut PnAG1 Gene Expression and Physiological Parameters

作　　者：周西[1,2] 周浩[1,3] 单世华[1] 李林[2] 杨晨[1,4] 刘宇[1,3] 闫彩霞[1] 张廷婷[1] 

机构地区：[1]山东省花生研究所,山东青岛266100 [2]湖南农业大学,长沙410128 [3]中国海洋大学,山东青岛266100 [4]东北农业大学,哈尔滨150030

出　　处：《中国农业科技导报》2012年第4期106-114,共9页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(30771361);"十一五"国家科技支撑计划项目(2009BADA8B03);现代农业产业技术体系建设专项资金项目(CARS-14)资助

摘　　要：为了探讨NBS-LRR结构域中抗性相关基因PnAG1与干旱胁迫的相关性,比较花生在不同干旱程度胁迫下主要防御酶活性变化情况以及PnAG1基因表达量变化动态。经干旱胁迫后测定不同时期两花生品种叶片的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)活性、丙二醛(MDA)含量和PnAG1基因表达量的动态变化。结果表明,在干旱胁迫后两品种的POD、GR、MDA变化均表现出先下降后上升的趋势,SOD活性则为表现为持续上升,而PnAG1基因的表达量变化同样也呈现出先下降后上升的趋势,与防御酶活性变化相似,因此推断PnAG1基因在抗病基础之上可能同植物防御体系存在相关。In this study, we studied on the pertinence between PnAG1 gene which relevant to NBS-LRR with drought stress, we compared the changes in peanut main defense enzyme activity and the dynamics of PnAG1 gene expression under different degrees of drought stress. After drought stress, leaf peroxidase （ POD ） activity, catalase （CAT） activity, supcroxide disrnutase （SOD） activity, glutathione oxidoreduetase （GR） activity, malondialdehyde （MDA） content and PnAG~ gene expression change dynamic of two peanut varieties during different periods were measured. The results showed that after drought stress the changes in POD, GR, MDA of two varieties showed a tendency of first rising then declined. The SOD activity was rising continuously, while the changes in PnAG1 gene expression also showed a trend of first decline then rise, similar to the changes in defensive enzyme activity. Therefore, conclusion was drew that the PnAGl genes might be interrelated with the plant defense system on the basis of the disease resistance.

关 键 词：花生 荧光定量PCR 酶活测定 NBS-LRR 干旱胁迫 

分 类 号：S565[农业科学—作物学] Q786[生物学—分子生物学]