温莪术根际亲和性高效促生菌的筛选和初步鉴定  

Screening and Preliminary Identification of Effective and Affinity Strains of Growth-promoting Rhizobacteria from Curcuma wenyujin Root

作　　者：刘祥亮[1] 周晓雷[1] 何文斐[2] 唐欣昀[1] 姜程曦[2] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]温州医学院药学院,浙江温州325035

出　　处：《中国农业科技导报》2012年第4期121-127,共7页Journal of Agricultural Science and Technology

基　　金：安徽省科技厅自然基金项目(090413082);"十二五"国家科技支撑计划项目(2011BAI04B04);浙江省教育厅科研项目(Y201120031)资助

摘　　要：为从温莪术根际筛选具有亲和性高效促生菌株,以温莪术凝集素为复筛工具,从温莪术根际促生菌中筛选能与温莪术凝集素相结合的亲和菌株。以甲壳素柱层析纯化,获得凝集素浓度为0.074 mg/mL,比活为8648.65 HU/mg。用纯化的凝集素对温莪术根际促生菌株进行凝集试验,得到能与温莪术凝集素产生特异性反应的菌株26株。定量检测26株亲和菌株的铁载体产量,发现其中13株铁载体产量极高,占亲和菌株的50%。对6株具有高温莪术凝集素亲和性和高铁载体产量的菌株进行初步鉴定,WEK0101、WEP0500为假单胞菌(Pseudomonas spp.);WE02、WEP0200为乳杆菌(Lactobacillus spp.);WEN0101为固氮菌(Azotobacterspp.);WEK0102为微球菌(Micrococcus sp.)。这6株温莪术亲和性根际促生菌具有制作温莪术高效特异性生物肥料的潜力。Lectin is purified from Wenyunjin （Curcuma wenyujin） and used as a tool to screen effective and affinity plant growth-promoting rhizobacteria strains, which can react with Wcnyunjin lcctin. Lectin can also be purified form caudex of Curcuma wenyujin by chitin column chromatography, and the specific concentration and specific activity of lectin are 0.074 mg/mL and 8 648.65 HU/mg, respectively. Forty four strains were isolated from Wenyunjin caudex and 26 strains could agglutinate positively with the Wenyunjin lectin. Among these 26 PGPR strains, 13 had high siderophore production, accounting for 50% of Wenyunjin PGPR strains isolated. Strains WEKO101 and WEP0500 are Pseudomonas spp. ; WE02 and WEP0200 Lactobacillus spp. ; WEN0101 Azotobacter spp. ; and WEK0102 is Micrococcus sp. These strains might be further evaluated for their high potential of specific bio-fertilizer of Wenyunjin. Six strains reacting positively with Wenyunjin lectin and high siderophore concentration were identified.

关 键 词：温莪术 凝集素 亲和性菌株 铁载体 

分 类 号：S154.39[农业科学—土壤学]