直接免疫荧光法对住院患儿肺炎衣原体的诊断意义  

Significance of direct immunofluorescence in diagnosis of Chlamydia pneumoniainfections in hospitalized infants

作　　者：胡芙蓉[1] 郭燕君 苗正友[3] 徐营 

机构地区：[1]嘉兴市第一医院检验科,浙江嘉兴314001 [2]嘉兴市医学院组胚教研室,浙江嘉兴314000 [3]嘉兴市妇幼保健院产前诊断中心实验室,浙江嘉兴314050

出　　处：《中华医院感染学杂志》2012年第15期3427-3429,共3页Chinese Journal of Nosocomiology

基　　金：嘉兴市科技局科研重点项目资助(2007AZ2013)

摘　　要：目的探讨一种快速诊断住院患儿呼吸道肺炎衣原体感染的方法。方法应用直接免疫荧光法,对因呼吸道感染而住院的婴幼儿鼻咽部分泌物肺炎衣原体抗原进行检测。结果 1022例婴幼儿中有82例肺炎衣原体检测阳性,阳性率为8.02%,其中男62例,阳性率为9.23%,女20例,阳性率为5.71%,差异有统计学意义(χ2=3.85,P<0.05);从年龄分布情况看,12～24月的婴幼儿感染率最高,达14.77%;4-6月为肺炎衣原体的高发季节,检出阳性率为12.74%。结论肺炎衣原体是婴幼儿呼吸道感染的重要病原体之一,应用直接免疫荧光法可以直接、快速对其进行诊断。OBJECTIVE To explore the rapid diagnosis of respiratory tract infection caused by Chlamydia pneumo- nia in hospitalized infants. METHODS Direct immunofluorescence was adopted to detect the antigen of C. pneumo- nia isolated from nasopharynx secretions of the infants who were hospitalized due to respiratory tract infections. RESULTS Of 1022 infants studied, C. pneumonia were detected positive in 82 cases with the positive rate of 8.02%, including 62 cases of male （9.23%） and 20 cases of female （5.71%）, the difference was statistically significant （x2= 3.85,P〈0.05）; the infants between 12 to 24 months of age had the highest infection rates, reaching to 14.77%; the period of Apr--Jun was the peak season for C. pneumonia infection with the detection rate of 12.74%. CONCLUSION C. pneumonia is one of the main pathogens causing respiratory tract infection in infants, direct immunofluorescence, as in a direct and rapid manner, can diagnose the C. pneumonia infections.

关 键 词：婴幼儿 肺炎衣原体 直接免疫荧光法 呼吸道 

分 类 号：R374[医药卫生—病原生物学]