化学发光免疫分析法在检测儿童EB病毒感染中的应用  

Detection of EB virus infections in children through chemiluminescent immunoassay

作　　者：汪光蓉[1,2] 王强[1] 凡瞿明[1] 何兰[1] 杜琴[1] 蔡艳娟[1] 毛明[1] 张国元[1] 

机构地区：[1]川北医学院附属医院检验科,四川南充637000 [2]川北医学院检验系,四川南充637000

出　　处：《中华医院感染学杂志》2012年第15期3430-3432,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨化学发光免疫分析法检测EB病毒抗体在儿童临床EB病毒(EBV)感染诊断中的价值。方法对228例符合EB病毒感染诊断标准的患儿采用化学发光免疫分析法检测血浆样本中VCA-IgM、EA-IgG、EB-NA-IgG、VCA-IgG含量。结果血浆VCA-IgM、EA-IgG、EBNA-IgG、VCA-IgG的检出率分别为84.65%、20.61%、25.88%、76.75%,差异具有统计学意义(P<0.05);各种抗体以不同组合方式出现VCA-IgM阳性分类中,以VCA-IgM+VCA-IgG组合模式最为多见,VCA-IgM阴性分类中,则以VCA-IgG+EBNA-IgG组合模式最为多见;不同年龄段儿童VCA-IgM的检出率差异有统计学意义(P<0.05),初次感染的年龄段主要集中在0～3岁。结论化学发光免疫分析法检测EB病毒抗体对儿童EB病毒感染的明确具有早期、全面、快速等优点,适合临床检测。OBJECTIVE To explore the clinical significance of chemiluminescent immunoassay in detection of Epstein-Barr virus infections. METHODS Plasma of 228 children with EB infections suited for diagnostics criteria was detected for VCA-IgM, EA-IgG, EBNA-IgG, and VCA-IgG by chemiluminescent immunoassay. RESULTS The positive rates of VCA-IgM, EA-IgG, EBNA-IgG, and VCA-IgG were 84. 65%, 20. 61%, 25. 88%, and 76.75 %, respectively, the difference was statistically significant （P〈0.05） ; among antibodies appeared as VCA- IgM positive classifications in a various combination way, VCA-IgM plus VCA-IgG positive was the most common; while among VCA-IgM negative, the VCA-IgG plus EBNA-IgG positive was the most common; the difference in the positive rate of VCA-IgM in the children of various age groups was statistically significant （P〈0.05） ; the primary infections mainly concentrated in the age group of 0-3 years of age. CONCLUSION EBV infections can be diagnosed in an early, comprehensive, and rapid manner by using chemiluminescent immunoassay which thus is suitable for clinical detection.

关 键 词：化学发光技术 EB病毒 儿童 

分 类 号：R373[医药卫生—病原生物学]