家蚕促咽侧体素受体基因克隆及发育表达  

Cloning and development expression of Allatotropin receptor gene in Bombyx mori

作　　者：邱娜莎[1] 刘畅[1] 严雅静[1] 魏兆军[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2012年第8期1117-1121,共5页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31072091);教育部新世纪优秀人才支持计划资助项目(NCET-07-0251)

摘　　要：文章克隆得到家蚕(Bombyx mori)神经肽促咽侧体素受体基因(Allatotropin receptor,Bommo-ATR),开放阅读框全长为1 254bp,编码416个氨基酸。Bommo-ATR氨基酸序列的二级结构预测为7次穿膜蛋白,符合GPCR家族成员的典型特征。实时荧光定量PCR结果表明,家蚕脑-咽下神经节复合体中的ATR mRNA在5龄幼虫期表达量最高,其次为蛹后期,蛹前期和成虫阶段表达量最低。其中5龄幼虫第2天表达量最高,1～5d持续维持较高的表达水平,这可能与保幼激素的合成有关。The Allatotropin receptor gene in Bombyx mori （Bommo-ATR） was cloned and sequenced. The open reading frame（ORF） was 1 254 bp in length, encoding 416 amino acids. The secondary structure of Bommo-ATR was predicted as seven trans-membrane proteins, which was in line with the typical characteristics of GPCR family members. The result of real-time quantitative PCR shows that the expression level of Bommo-ATR mRNA in Br-SG in 5th larval instar is the highest, the second is in the late pupal stage, then the early pupal stage and adult stage. The expression level of Bommo- ATR mRNA maintains high level from the 1st to the 5th day in 5th larval instar, with a highest level on the 2nd day, which may be related with the synthesis of juvenile hormone.

关 键 词：家蚕 促咽侧体素受体 发育表达 

分 类 号：Q781[生物学—分子生物学] Q786