AS患者外周血单个核细胞SCAMP2基因表达检测及其临床意义  

Expression and clinical significance of secretory carrier-associated membrane protein 2 in peripheral blood mononuclear cells of patients with atherosclerosis

作　　者：梁结玲[1] 陈立强[1] 李刚 王洋洋[1] 李海珠[1] 

机构地区：[1]广东省肇庆市第一人民医院检验科,526021 [2]广东吉赛生物科技有限公司,广州510663

出　　处：《国际检验医学杂志》2012年第9期1051-1052,共2页International Journal of Laboratory Medicine

摘　　要：目的研究SCAMP2在动脉粥样硬化(AS)患者外周血单个核细胞中的表达及其与疾病发生、发展的关系。方法分别采用半定量逆转录-聚合酶链反应(RT-PCR)和Westen blot检测23例AS患者和23例健康者SCAMP2mRNA和蛋白表达。结果 AS患者外周血单个核细胞SCAMP2mRNA和蛋白表达均明显高于健康者,差异有统计学意义(P<0.05)。结论 SCAMP2表达与AS发生存在一定相关性,在AS发病过程中可能起重要作用,为AS的诊断和预防提供了新线索。Objective To investigate the role of secretory carrier-associated membrane protein 2（SCAMP2）in the pathogenesis of atherosclerosis（AS）.Methods mRNA and protein level of SCAMP2 were detected by semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting respectively in peripheral blood mononuclear cells of 23 cases of patients with AS and 23 cases of healthy controls.Results mRNA and protein level of SCAMP2 in AS patients were statistically higher than those in healthy controls（P0.05）.Conclusion SCAMP2 might be involved in the pathogenesis of AS and could be a potential therapy or prevention target involved in the regulatory mechanisms in the development of AS.

关 键 词：动脉粥样硬化 载体蛋白质类 脂质体 胆固醇 SCAMP2 

分 类 号：R543.5[医药卫生—心血管疾病]