IL-12、IL-2、PHA协同诱导狼疮肾炎患者PBMC增殖和合成Ig的研究  

A Study on the Synergistic Induction by IL-12,IL-2 and PHA in the PBMC Proli feration of Patients with Lupus Nephritis and the Production of Ig Autoantibodies

作　　者：李清刚[1] 李幼姬[1] 李志坚[1] 曾丽霞[1] 

机构地区：[1]中山医科大学附属第一医院肾病研究所,广州510080

出　　处：《上海免疫学杂志》2000年第4期222-224,共3页Shanghai Journal of Immunology

基　　金：卫生部科学研究基金!(94 1 1 0 5 )资助项目

摘　　要：应用细胞培养技术 ,采用MTT和ELISA等方法 ,观察IL 12、IL 2、PHA体外协同诱导狼疮肾炎 (LN )活动期、静止期外周血单个核淋巴细胞 (PBMC )增殖和产生Ig自身抗体。结果表明 :与正常对照相比 ,IL 12与IL 2、PHA可明显刺激活动期、静止期PBMC增殖和产生大量IgG、IgM ,IgA无明显变化 ,且活动期强于静止期。单独应用IL 12对活动期PBMC作用最明显。提示 :IL 12可通过促进PBMC增殖产生大量自身抗体参与狼疮肾炎的发病。The effects of IL 12,IL 2 and PHA on the proliferation of PBMC in patients with lupus nephritis and their secretions of Ig antibodies were investigated in cell cultures by MTT and ELISA techniques.Results showed that IL 12 plus IL 2 or PHA,in comparison with the normal controls,could markedly promote the proliferation of PBMC in active and resting stages and the production of IgG and IgM autoantibodies,but showed no influence on the production of IgA antibody The effects were more prominent in active stage than in resting stage,and the application of IL 12 alone showed the most prominent effect in the active stage It suggests that IL 12 might participate the pathogenesis of lupus nephritis by promoting the proliferation of PBMC and the production of large amounts of autoantibodies

关 键 词：狼疮性肾炎 IL-12 IL-2 PBMC PHA 

分 类 号：R593.242[医药卫生—内科学]