叶酸对非洲爪蟾卵母细胞体外成熟的影响及作用机制  

Effect and the mechanism of folic acid on in vitro maturation of Xenopus oocytes

作　　者：高舒[1] 黄晓莉[1] 任晓梅[2] 陈俭静[1] 赵栋[1] 张伦[1] 吴坤[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150081 [2]陕西中医学院公共卫生系,陕西咸阳712046

出　　处：《中华疾病控制杂志》2012年第8期647-650,共4页Chinese Journal of Disease Control & Prevention

摘　　要：目的探讨叶酸(folic acid,FA)对非洲爪蟾卵母细胞体外成熟的影响及作用机制。方法将健康卵母细胞移入含不同浓度FA(分别为125μmol/L、250μmol/L、500μmol/L和1 000μmol/L)的ND96培养液中体外培养,同时设立阴性对照组和孕酮阳性对照组。加FA后每隔1 h观察1次细胞生发泡破裂(germinal vesicle break-down,GVBD),记录发生GVBD的卵母细胞数量,计算GVBD率,同时用10%三氯乙酸溶液固定后,切开进行确认。观察6 h后收集各组卵母细胞,采用蛋白免疫印迹实验(Western Blotting)检测CyclinB2,Mos,p-ERK1/ERK1蛋白的表达。结果除FA 125μmol/L、250μmol/L、500μmol/L、1 000μmol/L组2 h时与空白对照组GVBD率之间差异无统计学意义,其余各记录点的GVBD率与空白对照组间差异均有统计学意义(均有P<0.05);Western Blotting分析显示,FA和孕酮处理后卵母细胞的p-ERK1,CyclinB2,Mos蛋白表达水平明显升高。结论 FA可促进爪蟾卵母细胞体外成熟过程中GVBD发生,提示FA对爪蟾卵母细胞的成熟具有促进作用,其作用机制可能与干扰成熟促进因子和丝裂原活化蛋白激酶有关。Objective To investigate the effect of folic acid（FA） on oocyte maturation in vitro and the mechanism in Xenopus.Methods Healthy oocytes were cultured in ND96 with folic acid at dose of 125 μmol/L,250 μmol/L,500 μmol/L and 1 000 μmol/L in vitro,meanwhile,establishing negtive control and a positive control treated with progesterone.Then germinal vesicle breakdown（GVBD）status of the Xenopus oocytes were assessed at every 1 h,and confirmed by incision after fixation with 10%trichloracetic acid.Recording the numbers of oocytes and calculating the GVBD %.Oocytes were collected at 6h in each group,and the expression level of CyclinB2,Mos,p-ERK1/ERK1 were detected by Western Blotting.Results GVBD rate in folic acid-treated groups had significant difference compared with blank control（P〈0.05）,while there was no significant difference in 125 μmol/L,250 μmol/L,500 μmol/L,1 000 μmol/L groups at 2 h.Through Western Blotting analysis,p-ERK1,CyclinB2,Mos expression was increased significantly in oocytes treated with FA and progesterone.Conclusions Folic acid play a role in promoting GVBD in the maturation of Xenopus oocytes,which indicated that folic acid accelerates Xenopus oocytes matured process,and it was possibly due to the regulation of MPF and MAPK.

关 键 词：叶酸 卵母细胞 成熟促进因子 

分 类 号：R346.38[医药卫生—基础医学]