微透析技术观测不同麻醉深度的大鼠嗅球氨基酸类神经递质变化  

Monitoring the Contents of Glutamate and γ-aminobutyric Acid in Rat Olfactory Bulb in Different Depths of Anesthesia by Microdialysis

作　　者：刘飞[1,2] 陈荣祥[1,2] 李湘[1,2] 吴剑虹[3] 徐富强[1] 

机构地区：[1]中国科学院武汉物理与数学研究所,湖北武汉430071 [2]中国科学院研究生院,北京100049 [3]武汉大学化学与分子科学学院,湖北武汉430071

出　　处：《分析科学学报》2012年第4期465-468,共4页Journal of Analytical Science

摘　　要：本文探讨了使用微透析技术揭示麻醉的神经化学基础的可行性。以水合氯醛为麻醉剂,脑电记录监视脑活动状态,从处于不同麻醉深度的大鼠嗅球中获得微透析样品,经邻苯二甲醛衍生后,利用高效液相色谱-荧光检测对谷氨酸(Glu)和γ-氨基丁酸(GABA)进行了分离分析。结果表明:微透析样品中Glu含量随着麻醉程度的加深逐渐减少,但GABA的含量基本不受麻醉程度的影响,揭示了水合氯醛麻醉过程中对氨基酸类神经递质的影响,并提示微透析是一种可以用于研究麻醉机理和监控麻醉深度的分析技术。The mechanisms of anesthesia in different brain regions have not been clearly elucidated and there are not efficient technologies to monitor the anesthesia level quantitatively. This paper investigated the possibility of microdialysis to study the neurochemical mechanisms of anesthesia. Using electroencephalography to monitor the anesthesia depth of rats with chloral hydrate as anesthetics, microdialysis samples were collected from the olfactory bulb in different depths of anesthesia. The samples were analyzed by HPLC and fluorescence detection after derivatization of the amino acids with o -phthalaldehyde. The results demonstrated that the content of glutamate was decreased as the depth of anesthesia increased,while the content of ）＇-aminobutyric acid was not significantly affected by the depth of anesthesia. These findings revealed the neurochemical basis of chloral hydrate as an anesthetics and the feasibility of microdialysis as a tool to study the mechanism of anesthesia.

关 键 词：微透析 脑电记录 麻醉深度 神经递质 水合氯醛 

分 类 号：O657.72[理学—分析化学]