水稻叶色白化转绿及多分蘖矮秆基因hw-1(t)的图位克隆  

Map-Based Cloning of a Green-Revertible Albino and High-Tillering Dwarf Gene hw-1(t) in Rice

作　　者：郭涛[1] 黄永相[1] 黄宣[1] 刘永柱[1] 张建国[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学/国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《作物学报》2012年第8期1397-1406,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30771313);国家科技支撑计划项目(2008BAD97B02)

摘　　要：通过增加作图群体的样本量,将控制水稻白化转绿和多分蘖矮秆的基因hw-1(t)定位在第4染色体长臂2个InDel标记HW36和HW7之间24.9kb的物理距离内,该区域含有5个阅读框架。测序及酶切分析表明,突变体hfa-1仅在LOC_Os04g57320产生一个碱基(G→A)的突变,导致翻译提取终止,推断LOC_Os04g57320为hw-1(t)。BLAST分析发现hw-1(t)在水稻基因组中为单拷贝,与拟南芥AtIM和番茄SlPTOX基因同源性较高,但在转录子和蛋白质结构上存在一定差异;基因表达分析显示hw-1(t)属组成型表达基因,表达不受光照影响。In this study,the green-revertible albino and high-tillering dwarf gene hw-1（t） was fine mapped with a 24.9 kb physical distance between two InDel markers,HW36 and HW7 on chromosome 5,where five open reading frames were predicted.Sequencing and restriction enzyme digestion analysis revealed hfa-1 mutant carried a single nucleotide substitution（G→A） in the putative gene LOC_Os4g57320,which resulted in a premature stop codon.Therefore,we deduced that LOC_Os4g57320 is hw-1（t）.BLAST analysis indicated that hw-1（t） exits as a single copy in rice genome.Sequence alignment revealed that hw-1（t） was highly homologous to AtIM and SlPTOX,except for some variation on transcript and protein structure.Gene expression analysis indicated hw-1（t） was constitutive gene,which did not be affected by light.

关 键 词：水稻 白化转绿 多分蘖矮秆 hw-1(t) 克隆 

分 类 号：S511[农业科学—作物学]