海南逸生辣椒遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Capsicum in Hainan

作　　者：吕洁杰[1] 杨小波[1] 李东海[1] 汤华[2] 农寿千[3] 张孟文[1] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验园艺园林学院,海南海口570228 [2]海南大学热带作物种质资源保护与开发利用教育部重点实验农学院,海南海口570228 [3]海南省林业科学研究所,海南海口571100

出　　处：《贵州农业科学》2012年第7期21-24,共4页Guizhou Agricultural Sciences

基　　金：国家973计划前期预研专项(2010CB134512);海南大学植物学国家级重点学科资助"海南大学热带作物种质资源保护与开发利用"(2010hckled-06)

摘　　要：为了探明海南7个逸生辣椒品种的亲缘关系,为地方辣椒种质资源的合理利用与科学保护提供依据,采用ISSR分子标记技术对采自海南农村的7个逸生辣椒品种进行多态性和聚类分析。结果表明:1)从19对ISSR引物中筛选出12对进行PCR扩增,共扩增出50条DNA条带,多态性条带45条,多态率达87.22%。2)聚类分析将海南7个逸生辣椒品种分为2大类,簇生椒归为一类,其余6个品种的遗传距离相近,归为小米椒+朝天椒类。3)按海拔进行聚类分析发现,低海拔对辣椒的遗传多样性表现出规律性的变化。The genetic characters of seven Capsicum varieties in rural areas of Hainan Province were studied by ISSR marker to explore the genetic relationship and provide references for rational utilization and protection of local pepper germplasm resources. The results showed that： 1） 12 primers selected from 19 ISSR-primers were used for amplification and a total of 50 DNA bands were obtained, including 45 polymorphic bands（87. 220%）. 2） It was found by cluster analysis that the peppers could be classified into two major groups. The genetic distance of six varieties which had not clear differences except C. annuum var. fasciculatum were classified to one type （C. frutescens and C. annuum var. conoides）, while the C. annuum var. fasciculatum was of another type. 3） According to cluster analysis, the genetic diversity showed regular changes in low elevation zones.

关 键 词：ISSR分子标记 遗传多样性 逸生辣椒 海南 

分 类 号：S641.303.2[农业科学—蔬菜学]