南方根结线虫侵染对黄瓜植株生长和抗氧化系统的影响  

Effects of infection by Meloidogyne incognita on plant growth and antioxidant system of cucumber

作　　者：叶德友[1,2] 钱春桃[2] 王暄[3] 

机构地区：[1]甘肃省农业科学院蔬菜研究所,兰州730070 [2]南京农业大学园艺学院,南京210095 [3]南京农业大学植物保护学院,南京210095

出　　处：《植物保护学报》2012年第4期321-326,共6页Journal of Plant Protection

基　　金：国家自然科学基金重点项目(30830079);国家"863"项目(2010AA10Z108)

摘　　要：为探明野生黄瓜对南方根结线虫的抗性机制,采用温室盆栽苗期人工接种技术,研究了线虫侵染对黄瓜抗感品种植株生长和抗氧化系统的影响。南方根结线虫的侵染显著降低了感病品种津春4号的株高、叶面积、地上部鲜重与干重及根系活力,并显著增加其根系鲜重与干重,而对抗病品种西印度瓜的各项生长指标均未造成明显影响。西印度瓜中超氧阴离子(O.2-)、过氧化氢(H2O2)、电导率和丙二醛(MDA)含量增加幅度均小于津春4号,超氧化物歧化酶(SOD)活性降低,过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性增加幅度均显著高于津春4号。研究表明西印度瓜遭受的氧化胁迫较小,其抗氧化系统有效清除了氧化损伤。To understand mechanism of resistance to the root-knot nematode Meloidogyne incognita in wild cucumber, the effects of nematode infection on plant growth and antioxidant system of resistant and susceptible cucumber varieties were investigated with seedling inoculation in greenhouse. The results showed that plant height, leaf area, fresh and dry weight above ground and root activity were reduced, fresh and dry weight of root were increased significantly in susceptible Jinchun No. 4, while there is no obvious change for these indicators in resistant Xiyindugua after infection. The content of superoxide ani- on radical （ O2＂- ）, hydrogen peroxide （ H2 02 ）, malonicdialdehyde （MDA） and electrical conductivity were all increased with lower increasing rate in Xiyindugua than Jinchun No. 4. Superoxide dismutase （SOD） activity was reduced in Jinchun No. 4 while it increased in Xiyindugua, peroxidase （POD） and phenylalanine ammonialyase （PAL） activities were increased with higher increasing rate in resistant vari- ety than susceptible one. Oxidative stress induced by the pest was less in Xiyindugua than Jinchun No. 4 and the oxidative damage can be effectively removed by its antioxidant system.

关 键 词：南方根结线虫 黄瓜 植株生长 抗氧化 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]