重组人胸腺肽α_1对大鼠肝组织细胞色素P450基因表达的影响  

Effects of rh-thymosinα_1 on gene expression of hepatic cytochrome P450 in rats

作　　者：王兵 于琳 邓颖[2] 和凡[2] 黄民[2] 

机构地区：[1]深圳海王药业有限公司,深圳518057 [2]中山大学临床药理研究所,广州510080

出　　处：《世界临床药物》2012年第8期468-470,共3页World Clinical Drug

摘　　要：目的评价重组人胸腺肽α_1(rh-Tα_1)对大鼠肝组织细胞色素P450(CYP450)基因表达的影响。方法SD大鼠经腹腔注射不同剂量的rh-Tα_1(150、300和600μg/kg),14 d后从肝组织中提取总RNA,应用逆转录-聚合酶链反应(RT-PCR)检测CYP3A1、CYP1A2和CYP2E1 mRNA的表达。结果与空白对照组相比,rh-Tα_1可显著诱导CYP3A1、CYP 1A2和CYP 2E1的基因表达。结论rh-Tα_1通过诱导CYP450酶系的mRNA表达影响其活性。Objective To evaluate the effect of rh-thymosin α1（rh-Tα1） on gene expression of hepatic cytochrome P450（CYP450） in rats. Methods SD rats were administered intraperitonealy in a daily dose of rh-Tα1（150, 300 and 600 μg/kg） for 14 consecutive days. The levels of CYP 3A1, CYP1A2 and CYP2E1 mRNA in rats liver were analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. Results Compared with the blank group, the levels of CYP3A1, CYP1A2 and CYP2E1 mRNA were increased significantly compared with the blank control group. Conclusion rh-Tα1 can induce CYP450 （CYP3A1, CYP1A2 and CYP2E1） at the gene level.

关 键 词：重组人胸腺肽α1 细胞色素P450(CYP450) 逆转录-聚合酶链式反应 

分 类 号：R979.5[医药卫生—药品] R393[医药卫生—药学]