超快速液相色谱法测定新型“K2”香料中JWH-018的含量  

UFLC DETERMINATION OF THE CONTENT OF JWH-018 IN NEW “K2” SPICE

作　　者：徐鹏[1,2] 李晓娜[1] 刘克林[2] 凌笑梅[1] 卢炜[1] 

机构地区：[1]北京大学药学院,北京100191 [2]公安部物证鉴定中心,北京100038

出　　处：《中国药物依赖性杂志》2012年第4期282-284,共3页Chinese Journal of Drug Dependence

摘　　要：目的:建立测定新型"K2"香料中JWH-018含量的超快速液相色谱法。方法:采用Shim-pack XR-ODSⅡ色谱柱(2.0 mm×75 mm,2.2μm);流动相:乙腈(含0.1%甲酸)-水(含0.1%甲酸)(80:20,v/v),流速:0.25 mL·min-1;检测波长280 nm,柱温:35℃,进样量:2μL,外标法定量测定JWH-018的含量。结果:JWH-018的浓度在1-1000μg·mL-1范围内线性良好(A=8419.8C+13932,r=1.0000);最低检测限为90 ng·mL-1(S/N=3);低、中、高3种浓度的加样回收率(n=3)分别为101.0%(RSD=0.28%),101.0%(RSD=0.63%),101.7%(RSD=0.03%)。结论:本法操作简便、灵敏、准确,重复性好,适用于"K2"香料中JWH-018含量的测定。Objective： To establish an UFLC （ultra fast liquid chromatography ）method for determination of the content of JWH - 018 in new ＂ K2＂ spice. Methods： Shim - pack XR - ODS II chromatographic column （2.0 mm x 75 mm, 2. 2 μm） was used and the mobile phase consisted of acetonitrile and water（ 80：20, v/v）containing 0.1% formic acid. The mobile phase was pumped at a flow rate of 0.25 mL - min-1. The UV detection wavelength was set at 280 nm. The column temperature was ＂ K2 ＂ controlled at 35℃. The injection volume was 2 μL. The content of JWH -018 in new spice was calculated by external standardization method. Results：The calibration curve was linear with the range of 1 - 1000 μg · mL-1 （ A = 8419.8C ＋ 13932 ,r = 1.0（03）. The detective limit was 90 ng·mL-1 （ S/N = 3 ）. The mean recoveries（ n = 3 ） of low, middle and high concentrations were 101.0% （ RSD = 0.28% ）, 101.0% （ RSD = 0.63% ）, 101.7% （ RSD = 0.03% ） respectively. Conclusion： A simple, sensitive, accurate and reproducible UFLC method was established for the determination of JWH -018 content in new ＂K2＂ spice.

关 键 词：超快速液相色谱法 “K2”香料 JWH-018 含量测定 

分 类 号：R284.1[医药卫生—中药学]