基于BCNTs／GC电极的鸟嘌呤与腺嘌呤电化学行为及其同时检测  

Electrochemical Sensing of Guanine and Adenine Based on the Boron-Doped Carbon Nanotubes Modified Electrode

作　　者：夏雅淋[2] 邓春艳[1] 向娟[1] 

机构地区：[1]中南大学有色金属资源化学教育部重点实验室,化学化工学院,湖南长沙410083 [2]湖南大学化学生物传感与计量学国家重点实验室,化学化工学院,湖南长沙410082

出　　处：《电化学》2012年第4期365-370,共6页Journal of Electrochemistry

基　　金：国家自然科学基金项目(No.21005090);湖南大学化学生物传感与计量学国家重点实验室开放基金资助

摘　　要：利用掺硼碳纳米管(BCNTs)/GC电极研究了鸟嘌呤和腺嘌呤的电化学氧化行为.与GC和CNTs/GC电极相比,BCNTs/GC电极具有更强的电催化活性,且响应电流明显增加.两混合样品在BCNTs/GC电极上的氧化峰间隔较大,可实现对鸟嘌呤和腺嘌呤的同时检测.In the present paper, the boron-doped carbon nanotubes （BCNTs） modified glassy carbon （GC） electrode was simply fabricated, and the electrochemical oxidation behaviors of guanine and adenine at the BC- NTs/GC electrode were investigated. Compared with the bare GC and CNTs/GC electrodes, the BCNTs-mod- ified electrode exhibited extraordinary electrocatalytic activity towards the oxidations of guanine and adenine as indicated by the obvious increase in current responses. Moreover, the peak separation between guanine and ad- enine was large enough for their potential recognition in mixture without any separation or pretreatment. There- fore, the simultaneous determination of guanine and adenine was successfully achieved. The BCNTs/GC elec-trode showed high sensitivity, wide linear ranges and low detection limits for the electrochemical determination of guanine and adenine. The possibility of the BCNTs/GC electrode for the determination of guanine and ade-nine in calf thymus DNA has also been evaluated. The BCNTs/GC electrode has advantages of excellent cata-lytic activity, selectivity and simplicity, which plays a potential role in the development of the related DNA a-nalysis.

关 键 词：掺硼碳纳米管 鸟嘌呤 腺嘌呤 

分 类 号：O657.1[理学—分析化学]