王不留行对奶牛乳腺上皮细胞磷酸化雌激素受体的影响  

Effects of Semen Vaccariae on the phosphorylation estrogen receptor-α of bovine mammary gland epithelial cell

作　　者：李萌[1] 李楠[2] 佟慧丽[2] 万中英[2] 刘鹏[1] 迟涛[1] 王剑飞[1] 张娜[3] 刘莉莉[4] 卢雁[1] 宋戈[1] 高学军[2] 

机构地区：[1]东北农业大学黑龙江省乳品工业技术开发中心,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030 [3]东北农业大学乳品科学重点实验室,哈尔滨150030 [4]黑龙江中医药大学,哈尔滨150040

出　　处：《中国乳品工业》2012年第8期9-11,共3页China Dairy Industry

基　　金：黑龙江省青年科学基金资助项目(QC2010072)

摘　　要：通过研究王不留行对奶牛乳腺上皮细胞中磷酸化ERα的影响揭示王不留行促进乳腺泌乳的分子机制。应用Western blot和实时荧光RT-PCR检测P-ERα的变化;免疫荧光观察P-ERα定位表达变化。结果表明,中药王不留行增乳活性成分可通过调控奶牛乳腺上皮细胞雌激素信号转导途径中P-ERα的表达来增强其泌乳功能,但详细机制尚需进一步研究。In this experiment we detected the effects of Semen Vaccariae on the phosphorylation estrogen receptor-α of bovine mammary gland epithehal cell to reveal the galactopoietic molecular mechanism. Western blot and Quantitative real time PCR were put in use to study the change of p-erα expression, and co-localization expression of EtLα was observed by immunofluorescence. It suggests that Semen Vaccariae and DBP can promote the laction ability of DCMECs by regulating the expression ofp-erα in estrogen signal transduction pathway,however, the detailed mechanism needs further studies.

关 键 词：王不留行 邻苯二甲酸二丁酯 奶牛 乳腺上皮细胞 磷酸化雌激素受体 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]