奶牛乳腺中整联蛋白alpha6、beta4表达对细胞增殖的影响  

Effects on cell proliferaton and expression of integrin subunits α6 and β4 in cow mammary gland

作　　者：赵锋[1] 刘畅[1] 高学军[1] 李庆章[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,哈尔滨150030

出　　处：《中国乳品工业》2012年第8期12-15,共4页China Dairy Industry

基　　金：黑龙江省博士后启动基金(LBH-Z07235);东北农业大学博士科研启动基金

摘　　要：用组织免疫荧光和蛋白印迹检测两种整联蛋白亚基的定位和表达水平,并观察阻断它们的功能对乳腺上皮细胞增殖的影响。结果表明,奶牛乳腺中整联蛋白α6和β4主要在青春期和妊娠期的导管和腺泡的基底侧腺上皮细胞和肌上皮细胞表达,而脂肪细胞和成纤维细胞几乎无表达,α6亚基显示出基底侧极性分布,β4亚基在整个细胞膜均匀分布。阻断整联蛋白亚基功能会显著抑制细胞增殖,表明它们对于促进青春期和妊娠期导管和腺泡发育时的细胞增殖有着重要作用。We aimed to investigate expression and fuction of integrin α6 and β4 subunits in healthy Holstein cow mammary gland. Im- munofluorescence and Western blotting assay was carried out for integrin protein distribution and protein level, further their effects on mammary epithelial cells proliferation. Results showed that integrin α6 andβ4 both localized on the basal side membrane of ductal or alveolar glandular epithelial cells as well as myoepithelial cells in virgin and pregnant adult mammary gland, the former showing the distribution with basolateral polarity, and the latter which were detcted throughout the cell membrane, were also negative in adipocytes and fibroblasts. During perinatal and lactation integrin α6 andβ4 protein levels were relatively low. As key cell surface receptor subunits for laminin substrate in vitro, blocking their functions could significantly inhibit the prohferation of mammary epithelial cells. It concluded that the two integrin subunits promote cell proliferation for ducts or alveoli growth during puberty and pregnancy period.

关 键 词：奶牛 乳腺 整联蛋白 层粘连蛋白 细胞增殖 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]