金银花提取物多指标成分含量及指纹图谱同时检测研究  

Simultaneous Determination of the Contents and Fingerprint of Multi-Components in Honeysuckle Extract by HPLC

作　　者：何兵[1] 杨世艳[2] 张燕[3] 

机构地区：[1]泸州医学院药物研究中心,四川泸州646000 [2]泸州医学院附属中医医院超声影像科,四川泸州646000 [3]泸州医学院附属医院手麻科,四川泸州646000

出　　处：《中国药学杂志》2012年第16期1280-1284,共5页Chinese Pharmaceutical Journal

基　　金：泸州市2010年重点科技项目(泸市财企[2010]41号);泸州医学院2010年青年基金(泸医院[2010]108号)

摘　　要：目的建立金银花提取物中7种活性成分(新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸B、异绿原酸A及异绿原酸C)含量及指纹图谱同时检测的方法。方法以AkzoNobel Kromasil C18色谱柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸溶液梯度洗脱,测定波长326 nm,柱温30℃,流速1.0 mL.min-1。结果 7种成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率为97.98%～99.29%。结论该方法简便、灵敏、准确,可用于金银花提取物的质量控制。OBJECTIVE To establish a HPLC method for the simultaneous determination of the contents and fingerprint of 7 ac- tive constituents（ neoehlorogenic acid, ehlorogenie acid, cryptoehlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C ） in honeysuckle extract. METHODS The chromatographic separation was achieved on a AkzoNobel Kromasil Cls column（4. 6 mm× 250 mm,5 μm） , the mobile phase was acetonitrile-0. 1% phosphoric acid aqueous solution with gradi- ent elution at a flow rate of 1.0 mL · min- 1 , the detection wavelength was set at 326 nm, and the column temperature was 30 ℃. RE- SULTS All the 7 compounds showed good linearity in the ranges of the test concentrations. The RSDs of the precision, stability and reproducibility tests were less than 3%. The average recoveries were in the range of 97.98% -99.29%. CONCLUSION This meth- od is simple, sensitive, accurate, and can be used for quality control of honeysuckle extract.

关 键 词：HPLC 金银花提取物 指纹图谱 新绿原酸 绿原酸 隐绿原酸 咖啡酸 异绿原酸B 异绿原酸A 异绿原酸C 

分 类 号：R917[医药卫生—药物分析学]