荆芥内酯在大鼠体内的排泄研究  

Study on the Excretion of Schizonepetin in Rats

作　　者：姚卫峰[1] 耿婷[1] 丁安伟[1] 张丽[1] 

机构地区：[1]南京中医药大学,江苏省方剂高技术研究重点实验室,南京210046

出　　处：《中国药学杂志》2012年第16期1318-1323,共6页Chinese Pharmaceutical Journal

基　　金：国家科技重大专项课题:创新药物研究开发--候选药物研究(2009ZX09103-339)

摘　　要：目的研究荆芥内酯在大鼠体内的排泄情况。方法建立液-液萃取后HPLC测定大鼠尿液、粪便和胆汁中荆芥内酯含量的方法,并对大鼠灌胃/静脉注射荆芥内酯后的排泄情况和排泄特点进行分析。结果大鼠灌胃荆芥内酯(24 mg.kg-1)后,胆汁12 h内、尿液36 h内的排泄具有明显的雌雄差异(P<0.05),粪便36 h内的雌雄差异不明显(P>0.05);静脉注射荆芥内酯(12 mg.kg-1)后,尿液36 h内的排泄具有明显的雌雄差异(P<0.05),粪便36 h内的差异不明显(P>0.05)。结论荆芥内酯在大鼠尿液和胆汁中的排泄具有明显的雌性差异,以原型形式随尿液、粪便和胆汁排泄的量很少,可能主要以代谢产物的形式排出体外。OBJECTIVE To study the excretion profile of schizonepetin in rats. METHODS An HPLC-UV method was devel- oped and validated for the determination of schizonepetin in urine, feces and bile. Using this method, the excretion features were stud- ied in rats after oral and intravenous administration. RESULTS There was significant difference in the excretion percentages of sehi- zonepetin between male and female both in rat urine and bile for oral administration and in urine for intravenous administration. No sig- nificant difference was observed in the excretion of schizonepetin through feces for the two administration routes. CONCLUSION The amount of schizonepetin recovered from urine, feces and bile after administration is very low, indicating that schizonepetin is mainly ex- creted in the form of metabolites.

关 键 词：荆芥内酯 高效液相色谱法 排泄 尿液 粪便 胆汁 

分 类 号：R969.1[医药卫生—药理学]