应用SCoT标记分析玄参种质资源的遗传多样性  

Genetic diversity of Scrophularia ningpoensis based on SCoT analysis

作　　者：陈大霞[1] 张雪[1] 王钰[1] 李隆云[1] 

机构地区：[1]重庆市中药研究院中药种植研究所重庆市中药良种选育与评价工程技术研究中心,重庆400065

出　　处：《中国中药杂志》2012年第16期2368-2372,共5页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BAI13B02-2);重庆市重大科技攻关项目(CSTC;2009AA5052);重庆市重大专项(CSTC;2009AA5009)

摘　　要：目的:研究玄参种质资源的遗传多样性及亲缘关系。方法:应用目标起始密码子多态性(SCoT)技术对来源于全国各地的48份玄参种质进行遗传多态性分析。遗传距离采用TREECONW软件计算,UPGMA方法构建亲缘关系树状图。结果:28条引物共检测到279个位点,其中214个为多态位点,占76.7%。遗传距离变化范围在0.150 7～0.493 3。聚类结果显示栽培玄参亲缘关系较为复杂:有的种质具明显的地理相关性;有的种质聚类混杂,与地理分布无明显相关性;也有来源于同一地区的种质仅部分聚在一起,呈现出一定的地域性分布规律。结论:栽培玄参的遗传多样性水平较高,为优良品种的培育提供了丰富的遗传基础。Objective： To explore the genetic diversity and relationship of different Scrophularia ningpoensis cuhivars. Method： Forty-eight germplasmic resources of S. ningpoensis cuhivars were analyzed by Start Codon Targeted Polymorphism（SCOT） molecular markers. Genetic distance was calculated by TREECONW software and the systematic diagram of genetic relationship was clustered by UPGMA method. Result： A total of 279 bands were detected using 48 primers, among which 214 were polymorphic bands. The av- erage percentage of polymorphic bands was 76.7%. Genetic distance was changed from 0. 150 7 to 0.493 3. Clustering results showed that the genetic relationship of S. ningpoensis cuhivars was more complex. There was significant correlation between some germplasm and its geographic origin while geographical distribution of some germplasm was not very obvious, but it was also showed that some of the S. ningpoensis from the same region were in the same group which presented the law of geographical distribution in the tested materials. Conclusion： Significant polymorphism and genetic diversity can be observed among S. ningpoensis germplasm resources which provided a wealth of genetic basis for cultivating fine varieties.

关 键 词：玄参 SCoT 种质资源 遗传多样性 

分 类 号：R282[医药卫生—中药学] S567.53[医药卫生—中医学]