人参皂苷Rg_1诱导人白血病K562细胞株衰老的实验研究  

Experimental study on human leukemia cell line K562 senescence induced by ginsenoside Rg_1

作　　者：蔡世忠[1] 周玥[1] 刘俊[1] 刘典峰[1] 姜蓉[1] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室组织学与胚胎学教研室,重庆400016

出　　处：《中国中药杂志》2012年第16期2424-2428,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973818);重庆市科委自然科学基金重点项目(CSTC;2009BA5038)

摘　　要：目的:观察人参皂苷Rg1(Rg1)诱导人白血病K562细胞株衰老的作用及其机制。方法:MTT比色法检测Rg1对K562细胞增殖的影响,筛选最佳作用浓度及时间(20μmol.L-1,48 h)。流式细胞术检测Rg1对细胞周期的影响;SA-β-Gal染色检测细胞阳性染色百分率;RT-PCR法检测衰老相关基因p16,p53,p21,Rb的表达;电镜观察细胞衰老超微形态学改变。结果:Rg1在体外能明显抑制K562细胞增殖,使细胞阻滞于G2/M期;SA-β-Gal染色阳性细胞百分率显著增高(P<0.05);细胞衰老相关基因的表达上调(P<0.05);超微结构观察显示细胞增大,异染色质凝集、碎裂,线粒体体积增大,溶酶体体积增大、数目增多等衰老形态学变化。结论:Rg1能诱导K562细胞衰老,p53-p21-Rb,p16-Rb信号转导通路在其衰老调控中起重要作用。Objective： To observe the effect and mechanism of ginsenoside Rg1 in inducing senescence human leukemia K562 cell line. Method ： Proliferation of K562 cell line induced by Rg1 was detected by MTY colorimetric test for the purpose to screen optimal active concentration and time （20 μmol . L -1, 48 h）. Impact of Rg1 on cell cycle was analyzed using flow cytometry. The pe centage of staining positive cells was detected by SA-β-Gal staining. The expressions of senescence-related genes such as p16, p53, p21, Rb, were detected by RT-PCR and the changes in ultramicro-morphology were observed by transmission electron microscopy. Resuit： Rg1can significantly inhibit the proliferation of K562 cells in vitro and arrest the cells in G2/M phase. The percentage of positive cells stained by SA-β-Gal was dramatically increased （P 〈 0. 05） and the expression of cell senescence-related genes were up-regulated. The observation of ultrastructure showed that cell volume increase, heterochromatin condensation and fragmentation, mitochondrial volume increase, lysosomes increase in size and number. Conclusion ： Rg1 can induce the senescence of leukemia cell line K562 and play an important role in regulating p53-p21-Rb, pl6-Rb cell signaling pathway.

关 键 词：人参皂苷RG1 白血病 K562细胞株 细胞衰老 

分 类 号：R285.5[医药卫生—中药学] R733.7[医药卫生—中医学]