超声电子膀胱软镜在泌尿系疾病诊疗中的动物模型研究  

Feasibility of flexible ultrasonic cystoscopy in diagnosis and treatment for urinary tract diseases in vivo

作　　者：张振声[1] 许传亮[1] 吴承耀[1] 杨波[1] 罗睿[1] 王海峰[1] 王洋[2] 邓震[1] 唐亮[1] 孙颖浩[1] 

机构地区：[1]第二军医大学附属长海医院泌尿外科,上海200433 [2]第二军医大学附属长海医院病理科,上海200433

出　　处：《中华泌尿外科杂志》2012年第8期622-626,共5页Chinese Journal of Urology

摘　　要：目的探讨超声电子膀胱软镜应用于泌尿系疾病诊疗的可行性。方法采用1-2岁雌性巴马香猪4只为实验模型，以超声支气管镜为雏形，通过B超模式观察不同膀胱充盈量下膀胱各层结构声像图，通过彩色多普勒超声模式观察膀胱周围重要血管的声像图，初步总结超声电子膀胱软镜的操作技巧和难点。采用专用一次性抽吸式活检针进行内镜超声引导下分层定位穿刺活检，活检组织送病理以验证超声分层定位的准确性，探讨超声电子膀胱软镜下穿刺的有效性和安全性。结果超声膀胱镜可同步显示内镜图像和超声图像。香猪膀胱充盈90m1时，超声内镜下膀胱壁黏膜及黏膜下层、肌层、浆膜层层次清晰可辨并有助于穿刺针的分层定位，多普勒超声模式下，膀胱周围血管血流信号明显，超声引导穿刺可避免发生血管及周围脏器误伤。16处穿刺点送检组织病理结果与超声分层定位吻合。结论超声电子膀胱软镜诊断治疗泌尿系疾病安全可行，配套器械有待进一步改进。Objective To innovate and improve the feasibility of flexible ultrasonic cystoscopy （FUCS） in the diagnosis and treatment for urinary tract diseases in vivo by using female pigs as animal model. Methods Endobronchial ultrasonography was took as a substitute for FUCS and the operating skills and experience of FUCS in vivo by using female pigs as animal model were summarized, in order to testify the efficacy and safety of needle biopsy guided by FUCS. Results FUCS could simultaneously dis- play the endoscopic images and ultrasonic images. Ninety ml was suitable in pig bladder for FUCS when the layers of bladder wall were clear. The mucosa, submueosa, museularis and serosa layers were legible, which contributed to the needle positioning biopsy. Doppler ultrasound mode could distinguish the vessels, which could guide the puncture and avoid accidental injury of blood vessels and surrounding organs. Pathological results of positioning biopsy were consistent with FUCS evaluation for the three major layers of bladder wall. Conclusion Studies in vivo show that application of FUCS in the diagnosis and treatment for bladder disease is feasible, and further improvement of the FUCS equipment and clinical practice should be made.

关 键 词：电子膀胱软镜 膀胱 猪 模型 动物 

分 类 号：R445.1[医药卫生—影像医学与核医学]