改良ATB Fungus 3法检测糠秕马拉色菌对抗真菌药物的敏感性  

Antifungal susceptibility of Malassezia furfur in vitro tested by using modified ATB Fungus 3 method

作　　者：陈东科[1] 

机构地区：[1]卫生部北京医院检验科,北京100730

出　　处：《临床检验杂志》2012年第7期497-499,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(30872719)

摘　　要：目的用改良ATB Fungus 3法测定糠秕马拉色菌的药物敏感性,以了解临床患者呼吸道分离的糠秕马拉色菌对常用抗真菌药物的最低抑菌浓度(MIC)。方法在ATB F2半固体培养基中添加0.5%吐温40和0.5%吐温60,用ATB Fungus 3药敏板条对47株临床分离的糠秕马拉色菌进行MIC检测。结果氟康唑MIC50为>128μg/mL,MICRange为≤1.0～>128μg/mL;5-氟胞嘧啶MIC50为>16μg/mL,MICRange为>16μg/mL;沃尔康唑MIC50为8.0μg/mL,MICRange为≤0.06～>8.0μg/mL;两性霉素B MIC50为4.0μg/mL,MICRange为≤0.5～16μg/mL;伊曲康唑MIC50为1.0μg/mL,MICRange为≤0.125～4.0μg/mL。呼吸道分离的糠秕马拉色菌对5种抗真菌药物的MIC值高于健康人皮肤分离的糠秕马拉色菌及标准菌株ATCC14521。结论改良ATB Fungus 3法操作简便,结果重复性好且易观察。糠秕马拉色菌MIC值的升高可能与患者使用抗真菌药物有关。Objective To determine the drug-susceptibility of Malassezia furfur by using modified ATB Fungus 3 method and evaluate the minimum inhibitory concentration （MIC） of clinical isolates of Malassezia furfur to antifungal agents. Methods Tween-40 and Tween-60 were added into ATB F2 semisolid culture media with final concentration of 0.5%. The MICs of 5 antifungal agents against 47 strains of Malassezia furfur were determined by ATB Fungus 3 method. Results The MIC50 of fluconazole, 5-flucytosine, voriconazole, amphotericin B and itraconazole against 47 Malassezia furfur strains were more than 128, more than 16, 8. 0, 4.0 and 1.0 μg/mL respectively, and the ranges of MIC were less than or equal tol. 0 to more than 128 μg/mL, more than 16 i.μg/mL, less than or equal to 0.06 to more than 8.0 μg/mL, less than or equal to 0.5 to 16 μg/mL, less than or equal to 0. 125 to 4.0 μg/mL. The results showed that the MIC values of 5 antifungal agents against clinical isolates of Malasseziafurfur from respiratory tract were ~igher than those of typical strain ATCC 14521 and the Malasseziafuffur strains isolated from normal human skin. Conclusion The improved ATB Fungus 3 method with good reproducible results should be easily available for the determination of drug-susceptibility of Malassezia fuffur. The high MICs of antifungal agents against clinical isolates of Malasseziafugrur may be due to increased application of antifungal agents.

关 键 词：糠秕马拉色菌 ATB FUNGUS 3法 抗真菌药物 最低抑菌浓度 

分 类 号：R446.5[医药卫生—诊断学]