携IL-8单抗靶向超声造影剂与血管内皮细胞相互作用的实验研究  

Study on interactions between targeted ultrasound contrast agents with anti-human interleukin-8 monoclone antibody and activated vascular endothelial cellls

作　　者：杨薇[1] 陆永萍[1] 袁媛[1] 周虹[2] 刘蕊[1] 荆治[1] 汤跃跃[1] 

机构地区：[1]昆明医学院第四附属医院暨云南省第二人民医院超声科,650021 [2]昆明医学院第四附属医院暨云南省第二人民医院病理科,650021

出　　处：《中华超声影像学杂志》2012年第8期715-718,共4页Chinese Journal of Ultrasonography

基　　金：云南省科技厅-昆明医学院联合专项基金（2009CD189）;昆明医学院研究生创新基金（2011S02）

摘　　要：目的观察自制携带IL-8单抗靶向超声造影剂与损伤血管内皮细胞的相互作用，探讨IL-8在粥样斑块形成过程中的作用和评价血管内皮功能的新方法。方法采用交联剂将抗人IL-8单克隆抗体偶联到SonoVue微泡表面，制备靶向性超声微泡；倒置显微镜下分别观察SonoVue微泡、靶向性微泡与正常内皮细胞、损伤内皮细胞的结合作用，高倍视野下计数内皮细胞及黏附的微泡数，通过计算微气泡与内皮细胞的比值对两者之间的结合作用进行定量分析。结果仅有极少量对照组SonoVue微泡黏附于正常及损伤内皮细胞；而镜下可见携IL-8单抗的SonoVue微泡黏附于内皮细胞，黏附于损伤内皮细胞表面的微泡数显著高于正常内皮细胞。结论携IL-8单抗的靶向性超声造影剂能够特异性结合在损伤细胞表面，拓展了超声成像技术检测血管内皮损伤、评价血管内皮功能的新领域。Objective To observe the interactions between a anti-interleukin-8 monoctonal antibody （anti-IL-8 mAb） carried targeted ultrasound contrast agents and the injured vascular endothelial cells, as well as to explore the role of IL-8 in the formation of atherosclerotic plaques and a new assessing method of vascular endothelial functions. Methods The targeted ultrasound contrast agent was prepared by using a crosslinking agent to couple a anti-IL-8 mAb with SonoVue microbubbles. The interactions between SonoVue microbubbles/the targeted microbubbles and normal/injured endothelial cells were observed under an inverted microscope, respectively. The numbers of endothelial cells and adhered microbubbles were counted under high power magnification. The ratio of microbubbles to endothelial cells was calculated for the quantitative analysis of the interactions. Results In the control group, only a slight amount of original SonoVue microbubbles were bound to normal/injured endothelial cells. In contrast,it was visible under the microscope that the anti-IL-8 mAb carried SonoVue could be bound to endothelial cells,and the number of microbubbles bound to the surface of injured endothelial cells was significantly higher than that bound to the normal endothelial cells. Conclusions The anti-IL-8 mAb carried targeted ultrasound contrast agent could be readily bound to the surface of the injured cells specifically, and thus suggesting a new direction for ultrasonic detection of vascular endothelial injury and the ultrasonic assessment of vascular endothelial functions.

关 键 词：超声检查 微气泡 内皮细胞 白细胞介素-8 

分 类 号：R445.1[医药卫生—影像医学与核医学]