超声击破微泡对犬睾丸组织及细胞影响的研究  

Preliminary study of effects on dogs＇ testis by microbubbles excitated by ultrasound

作　　者：萧淑宜[1] 柳建华[1] 区文财[1] 何景光[1] 邱春花[1] 马穗红[1] 杜洪[1] 

机构地区：[1]广州市第一人民医院功能检查科,510180

出　　处：《中华超声影像学杂志》2012年第8期723-726,共4页Chinese Journal of Ultrasonography

基　　金：广州市科技计划项目（2009J1-C501-1）

摘　　要：目的探讨超声击破微泡对犬睾丸及组织细胞的影响。方法16只雄性成年犬的16个睾丸，随机分为3组，微泡（MB）组4个，治疗超声（TUS）组和微泡超声（MEUS）组各6个，依分组分别施以静脉注射微泡、超声治疗和微泡联合超声治疗后进行光镜和电镜检查。结果MEUS组光镜下生精上皮层次明显减少，生精细胞排列紊乱，出现明显空泡，组织间隙微血管充血，管壁破损，红细胞溢出，部分生精小管内见红细胞；电镜下细胞间隙增宽，生精小管基膜局部断裂，支持细胞间的紧密连接消失。TUS组生精上皮层次稍减少，部分生精细胞出现空泡；基膜未见明显改变，组织间隙内偶见毛细血管充血，支持细胞间的紧密连接疏松。MB组未见明显变化。结论超声击破微泡可打开血睾屏障，为睾丸疾病靶向性治疗提供依据。Objective To explore the effects on dogs＇ testis hy microbubbles excitated by ultrasound. Methods Sixteen male dogs were divided into 3 groups randomly. Pulsed ultrasound irradiation and intravenous microbubbles injection were both applied in the microbubble enhanced ultrasound group （MEUS）,pulsed ultrasound irradiation and intravenous microbubbles injection were individually applied in the ultrasound group （TUS） and the simple microbubbles group （MB）. Results In MEUS group,the layers of seminiferous tubules decreased and the germ cells were arranged disorderly and bubbles appeared in the cells. The appearances of focal ultra-structural damage such as intercellular space widening, baseline broken and tight-connection of sustentaeular cells disappearing were observed by transmission electron microscope, which were slightly or hardly demonstrated in other groups. Conclusions Microbubbles excitated by ultrasound can affect testicular germ cells and the integrity of bloold-testis barrier, which providing new train of thought of target treatments of testis diseases.

关 键 词：超声疗法 微气泡 睾丸 血睾屏障 

分 类 号：R310[医药卫生—基础医学]