原发性干燥综合征患者单核源性树突状细胞对聚肌苷酸胞苷酸刺激的反应性  

Monocyte-derived dendritic cells from primary Sjgren′s syndrome patients were hypersensitive to polyinosinic polycytidylic acid stimulation

作　　者：谷明莉[1] 孙懿[1] 秦琴[1] 黄元兰[2] 陈孙孝[3] 邓安梅[1] 

机构地区：[1]第二军医大学附属长海医院实验诊断科,上海200433 [2]第二军医大学附属长征医院实验诊断科,上海200003 [3]第二军医大学附属长征医院皮肤科,上海200003

出　　处：《广东医学》2012年第15期2233-2236,共4页Guangdong Medical Journal

基　　金：国家自然科学基金面上项目(编号:30972730);上海市科学技术委员会科研计划项目(编号:09JC1405400;11JC1410902)

摘　　要：目的观察原发性干燥综合征(pSS)患者外周血单核源性树突状细胞在聚肌苷酸胞苷酸(polyI∶C)刺激下释放干扰素等炎性因子的能力。方法用白细胞介素(IL)-4和粒细胞-巨噬细胞集落刺激因子从pSS患者外周血单核细胞中诱导出树突状细胞,并用50μg/mL polyI∶C进行刺激,在刺激后第6、12、24小时以ELISA法检测细胞培养基上清液中干扰素(IFN)-α、IFN-β、肿瘤坏死因子-α(TNF-α)、IL-1β、IL-6、IL-8、IL-12和IL-23的浓度,以RT-PCR检测树突状细胞内Toll样受体3(TLR3)分子的表达。结果在polyI∶C作用下,pSS患者单核源性树突状细胞释放IFN-α、IFN-β及TNF-α等因子的能力显著强于来自健康个体的单核源性树突状细胞。虽然pSS患者及健康个体单核源性树突状细胞内TLR3的表达在polyI∶C刺激作用下均随时间逐渐降低,但pSS患者TLR3的降低相对缓慢。结论 pSS患者单核源性树突状细胞对polyI∶C刺激呈现高反应性,且这种高反应性可能与TLR3信号作用异常有关。单核源性树突状细胞的这种特征可能与pSS的发病机制有关。Objective To investigate the potential correlation between pathogenesis of primary Sjrgren＇s syndrome （pSS） and the funetion of monocyte -derived dendritic cells. Methods Monocytes from 11 pSS patients and 11 healthy controls were isolated and induced to dendritic cells in the presence of IL - 4 and GM - CSF. Polyinosinic polycytidylic acid （polyI： C, 50 μg/mL） was used to simulate the monoeyte -derived dendritic cells. Serial cytokines and TLR3 mRNA were assessed by ELISA and RT -PCR, respectively, at the 6th, 12th and 14th hour after polyI： C treatment. Results Significantly more IFN - α, IFN -β and TNF - α were produced in monocyte - derived dentritic cells from pSS patients when challenged with polyI： C. Although TLR3 mRNA expression was down -regulated by polyI： C stimulation, it was impaired in pSS patients. Conclusion The Hypersensitivity to polyI： C, which may associated with TLR3 abnormality, of the monocyte - derived dendritic cells from pSS patients was identified. The abnormality of monoeyte - derived dendritic cells from pSS patients may involved in the pathogenesis of pSS.

关 键 词：原发性干燥综合征 单核源性树突状细胞 天然免疫 聚肌苷酸胞苷酸 TOLL样受体 细胞因子 

分 类 号：R512.62[医药卫生—内科学]