蝴蝶兰细菌性软腐病病原鉴定  

Identification of the pathogen causing soft rot of Phalaenopsis amabili

作　　者：肖爱萍[1] 游春平[1] 向梅梅[1] 

机构地区：[1]仲恺农业工程学院农学院,广东广州510225

出　　处：《广东农业科学》2012年第15期74-75,共2页Guangdong Agricultural Sciences

基　　金：广东省现代农业产业技术体系花卉病虫害防治创新团队建设项目(2060302)

摘　　要：从广州地区发生的蝴蝶兰软腐病病叶上分离病原菌并进行分子(16S rDNA)鉴定,结果显示,病原菌与洋葱伯克赫尔德氏菌(Burkholderia cepacia)和新洋葱伯克赫尔德氏菌(Burkholderia cenocepacia)的16S rDNA同源性达99.76%,进一步通过分子系统发育树分析,广州地区蝴蝶兰软腐病菌与洋葱伯克赫尔德氏菌为同一宗族(clan),表明此病原菌为洋葱伯克赫尔德氏菌。Identification of the pathogen causing soft rot of Phalaenopsis amabili was performed by 16S rDNA sequence analysis and phylogenic tree construction, the result indicated that, the 16S rDNA homology between the pathogen and Burkholderia cepacia or Burkholderia cenocepacia was over 99%; further analysis was carried out by phylogenic tree construction of 16S rDNA sequences from those homological bacteria, the result showed that the pathogen and Burkholderia cepacia belonged to the same clan.

关 键 词：蝴蝶兰细菌性软腐病 病原菌鉴定 16S rDNA同源性 系统发育树 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]