重组大肠杆菌菌蜕与幽门螺杆菌蛋白质疫苗协同免疫BALB/c小鼠  

Cooperative Vaccination of BLAB/c Mice with Bacterial Ghosts Dis playing UreB Epitope of Helicobacter pylor and Recombinant Pro tein LTBKAT

作　　者：张瑞平[1] 刘纯杰[2] 陶好霞[2] 张兆山[2] 

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2012年第4期506-510,514,共6页Letters in Biotechnology

摘　　要：目的:考察重组菌蜕和蛋白质疫苗混合后对BAB/c小鼠机体免疫反应的影响。方法:首先构建展示尿素酶B抗原表位的大肠杆菌菌蜕,同时通过融合PCR构建分子内佐剂蛋白质疫苗rLTBKAT,然后分别利用该菌蜕和蛋白质疫苗免疫BAB/c小鼠。结果:当rLTBKAT和重组菌蜕联合口服免疫BAB/c小鼠时,其抗尿素酶B抗体的效价由单独免疫时的1∶(239±23)提高到1∶(681±76),二者差异极显著(P=0.009);而其抗过氧化氢酶抗体的效价则由单独免疫时的1∶(2800±275)下降至1∶(1800±400),二者差异不显著(P=0.08)。抗体分型试验表明,单独和联合免疫时,其抗尿素酶B抗体类型均以IgG1为主,而抗过氧化氢酶抗体类型则由单独免疫时的IgG1为主转变为联合免疫时的IgG2a为主。联合免疫时,小鼠抗尿素酶B和抗过氧化氢酶IgA抗体水平均高于单独免疫时。结论:重组菌蜕和蛋白质疫苗联合免疫,可提高机体对某些抗原的免疫水平或改变其反应类型。Objective： To study the immune response of BLAB/e mice to vaccination of recombinant bacterial ghosts cooperated with protein vaccine. Methods： The bacterial ghost displaying UreB epitope as well as recombi- nant LTBKAT were constructed, and then they were mixed or separately orally administrated by BLAB/c mice. Results： The anti-UreB antibody titer of mice immunized with mixed vaccine was 1：（681±76）, which was significantly higher than that immunized with single bacterial ghosts[1： （239±23）]. The anti-UreB antibody type of both groups was mainly IgG1. The anti-KatA antibody titer of mice immunized with mixed vaccine was 1：（1800±400）, which was lower than that immunized with single protein vaccine[1： （2800±275）]. The anti-KatA antibody type of mixed vaccination was IgG2a, which was very different from that of protein vaccination. The anti-UreB and anti-KatA IgA antibody level of mixed vaccination were both higher than those of single immunization. Conclusion： Immunogenicity level of BLAB/c mice was improved, even immunogenicity type was changed.

关 键 词：蛋白质疫苗 菌蜕 幽门螺杆菌 递送系统 

分 类 号：Q78[生物学—分子生物学] R392.1[医药卫生—免疫学]