PCR鉴定肺炎克雷伯菌的强毒性血清型  

Polymerase Chain Reaction Analysis for Detecting Highly Virulent Serotypes of Klebsiella pneumoniae

作　　者：和晋渝[1] 邱景富[1] 刘梦颖[1,2] 闫小娟 李迎丽[1,2] 郭兆彪[2] 周冬生[2] 

机构地区：[1]重庆医科大学公共卫生与管理学院,重庆400016 [2]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《生物技术通讯》2012年第4期554-557,共4页Letters in Biotechnology

基　　金：国家自然科学基金(31071093;31170129);国家重点基础研究发展计划(2009CB522600)

摘　　要：目的:了解肺炎克雷伯菌强毒性血清型K1、K2、K54和K57型菌株在我国重庆、北京、深圳三地的分布及流行趋势。方法:采用PCR对310株肺炎克雷伯菌临床分离株进行血清型K1、K2、K54和K57检测。结果:310株菌中,K1、K2、K54和K57血清型分别占14.2%、9.4%、6.5%和4.2%;来自呼吸系统标本分离株中的K1血清型菌株在4种检测的强毒血清型中占首位,为呼吸系统总数的17.4%。结论:310株肺炎克雷伯菌的4种强毒性血清型中,K1血清型菌株所占比例高,较为流行。Objective： To characterize the distribution of highly virulent serotypes of Klebsiella pneumoniae in Chongqing, Beijing and Shenzhen city in China. Methods： Genomic DNA was extracted, and then four serotypes related to the highly virulent strains were screened in a collection of 310 strains of K.pneurnoniae by polymerase chain reaetion（PCR）. Results： Serotypes K1, K2, K57 and K54 accounted for 14.2%, 9.4%, 6.5% and 4.2% of the 310 isolates tested. Compared to the other three serotypes, the serotype K1 occurred most frequently in the strains from the respiratory system. Conclusion： K1 serotype is predominant in the 310 strains of K.pneurnoniae in relative to the other serotypes tested.

关 键 词：肺炎克雷伯菌 血清型 强毒株 聚合酶链反应 

分 类 号：Q78[生物学—分子生物学] R517.6[医药卫生—内科学]