p57kip2在神经管发育过程中的表达差异  

Expression of p57kip2 in neural tube tissues in embryonic mice with genetic neural tube defect and its meaning

作　　者：李新军[1] 韩杨云[1] 徐宏[1] 杨忠[2] 曾义[3] 孙中书[1] 李红丽[2] 游潮[4] 

机构地区：[1]德阳市人民医院神经外科,四川618000 [2]第三军医大学神经生物教研室,重庆400038 [3]四川省人民医院神经外科,成都610041 [4]四川大学华西医院神经外科,成都610041

出　　处：《中国临床神经外科杂志》2012年第8期479-481,共3页Chinese Journal of Clinical Neurosurgery

基　　金：四川省卫生厅科技攻关项目(No.080128)

摘　　要：目的探讨细胞增殖抑制因子p57kip2在神经管发育过程中的作用。方法根据Klootwijk等方法制作神经管缺陷(NTD)小鼠模型,采用基因芯片技术比较正常与NTD小鼠怀孕9.5d和10.5d胚胎的神经管组织中p57kip2基因表达的差异,并对其进行Northern杂交验证。结果基因芯片结果显示,小鼠正常神经胚围形成期(孕9.5和10.5d)p57kip2表达显著上调,在维甲酸诱导致NTD的神经管组织中,包括怀孕9.5d和10.5d两个时相,p57kip2表达均呈现下调趋势。Northern杂交验证结果均显示其变化趋势与芯片结果相同。结论 p57kip2可能参与正常神经管关闭的生理过程,在神经系统发育中发挥重要作用。Objective To investigate the role of the cell proliferation inhibition factor p57kip2 in neural tube development. Methods The mouse model for human neural tube defects （NTD） was established by Klootwijk et al method. The expression of p57kip2 in the neural tube tissues of the embryos aged 9.5 days and 10.5 clays of 28 mice with genetic NTD was determined by eDNA microarray gene hybridization technique and p57kip2 gene was verified by Northern hybridization. The expression of p57kip2 in the neural tube tissues of embryos aged 9.5 clays and 10.5 clays of 28 normal mice served as control. Results The expression of p57kip2 in the neural tube tissues was significantly up-regulated in the normal mice, but it was significantly down-regulated in the mice with genetic NTD around the normal neurnlation phase. The above-mentioned changes in p57kip2 expression were confirmed by Northern hybridization. Conclusion It is suggested that p57kip2 may play an important role in nervous system development through its participation in the physiological process of normal neural tube closure.

关 键 词：神经管缺陷 神经胚形成 P57KIP2 基因芯片 维甲酸 

分 类 号：Q344.13[生物学—遗传学]