婴儿捂热综合征外周血单个核细胞核因子-κB活化及血清白细胞介素-17等炎症因子变化的临床意义  

The clinical significance of nuclear factor-kB activation in peripheral blood mononuclear cells, serum level of interleukin-17 and serum levels of other correlated inflammatory cytokines in infant muggy syndrome

作　　者：邹峥[1] 刘小惠[1] 段君凯[1] 敖建云[2] 柯江维[3] 刘志强[3] 吴卫萍[1] 

机构地区：[1]江西省儿童医院风湿免疫科,南昌330006 [2]南昌大学第二附属医院检验科,南昌330006 [3]江西省儿童医院检验科,南昌330006

出　　处：《中国小儿急救医学》2012年第4期364-367,共4页Chinese Pediatric Emergency Medicine

基　　金：国家十一五科技支撑项目（2008BAI68B07）

摘　　要：目的探讨外周血单个核细胞核因子（nuclearfactor，NF）-kB活化状态及血清炎症细胞因子在婴儿捂热综合征（infantmuggysyndrome，IMS）中的临床意义。方法2008年1月至2011年1月采用酶联免疫吸附法检测100例IMS患儿及32例健康儿童（对照组）外周血单个核细胞NF-kB活化率及白细胞介素（interleukin，IL）-17、IL-6、肿瘤坏死因子（tumornecrosisfactor，TNF）-α和IL-10血清水平。同时采用流式细胞法检测其中46例IMS患儿及32例对照组儿童NF—KB阳性率，并分析以上指标与多器官功能障碍综合征（multipleorgandysfunctionsyndrome，MODS）的关系。结果与对照组相比，100例IMS患儿采用酶联免疫吸附法检测的NF—KB活化率[（11．042±6．792）％VS（4．528±1．378）％]及46例IMS患儿采用流式细胞法检测的NF—kB阳性率[（28．780±13．820）％VS（7．078±5．395）％]均明显升高（P均〈0．01）。IMS患儿血清IL-17、IL-6、IL-10水平均显著高于对照组（P均〈0．01），血清TNF-α水平稍高于对照组，但差异无统计学意义（P〉0．05）。IMS合并MODS患儿NF—KB活化率[（14．591±7．626）％VS（8．576±4．851）％I、NF-kB阳性率1（36．087±12．056）％VS（23．590±11．263）％]及IL-17、IL-6、TNF—α和IL-10水平均显著高于不合并MODS者（P〈0．01）。结论外周血单个核细胞NF—kB活化及血清IL-17、IL-6水平对IMS患儿的缺氧性炎症损伤起重要作用。NF—kB高度活化及IL-17、IL-6、TNF-α高度活化与IMS患儿发生MODS有关。血清IL-10水平增高未能阻止IMS患儿的缺氧性炎症损伤。Objective To investigate the clinical significance of nuclear factor （NF）-KB activation in peripheral blood mononuclear cells （PBMCs） and the serum levels of correlated inflammatory cytokines in children with infant muggy syndrome（IMS）. Methods Blood samples from 100 patients with IMS and those from 32 Healthy infants（ control group）were detected by ELISA for amount of NF-KB activation in PBMCs and for serum levels of interleukin（IL）-17, IL-6, tumor necrosis factor （TNF）-α and IL-10 respectively from Jan 2008 to Jan 2011. At the same time,blood samples from 46 out of the above 100 patients with IMS and those from the 32 controls for positive rate of activation of NF-kB in PBMCs were detected by flow cytometry as well. The relationship between all the data and multiple organ dysfunction syndrome （ MODS ） were analyzed re- spectively. Results As compared with that of control group, the percentage of activated NF-kB in PBMCs in 100 patients with IMS detected by ELISA [ （ 11. 042 ± 6. 792 ） % vs （ 4. 528 ± 1. 378 ） % ] and the positive rate of NF-KB activation in 46 patients with IMS detected by flow cytometry [ （ 28.780 ± 13. 820 ） % vs （7. 078± 5. 395 ） % ] were both significantly higher （ P 〈 0. 01 ）. The serum levels of IL-17, IL-6 and IL-10 were also significantly higher in patients with IMS than those in control group（ P 〈 0. 01 ）. The serum level of TNF-α was higher in patients with IMS than that in control group but without significance （ P 〉 0. 05 ）. The percentage of activated NF-KB [ （ 14. 591 ±7. 626） % vs （8. 576±4. 851 ） % ] ,the positive rate of NF-KB ac- tivation[ （36. 087 ± 12. 056）% vs （23. 590±11. 263 ）% ], and the serum levels of IL-17, IL-6, TNF-α and IL-10 were all significantly higher in IMS patients with MODS than those in IMS patients without MODS （P 〈 0.01 ）. Conclusion The inflammatory factors of NF-KB activation in PBMCs and the high serum levels of IL-17 and IL-6 are potent to cause inflammatory

关 键 词：捂热综合征 婴儿 核因子-KB 细胞因子 

分 类 号：R720.597[医药卫生—急诊医学]