紫穗槐种子内生根瘤菌数量分布与种类特征  

Amount distribution and category characteristics of endogenous rhizobium in Amorpha fruticosa seeds

作　　者：陈利云[1] 周志宇[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《干旱地区农业研究》2012年第4期231-235,共5页Agricultural Research in the Arid Areas

基　　金：国家科技支撑计划资助项目(2007BAD80B05);国家自然科学基金资助项目(30800801)

摘　　要：为探明根瘤菌在植物种子内的分布规律和种类特征,对紫穗槐种子荚果皮、种皮、子叶和胚芽4个部位内生根瘤菌的分布数量与菌株种类进行了研究。结果表明:内生根瘤菌在紫穗槐种子不同部位数量分布有很大差异,以种皮最丰(6.663×106cfu/pod),占种子内生根瘤菌总数的50%,荚果皮次之(3.335×106cfu/pod),子叶和胚芽内仅有少量分布(2.087×106cfu/pod,1.250×106cfu/pod)。分离纯化的32株根瘤菌RAPD检测图谱显示为5个不同类型,以B型图谱菌株数目最多,为13株,占菌株总数的40.62%,C型菌株8株,D、A和E型图谱菌株分别为6、4株和1株,其中种皮16个菌株电泳图谱为5种,荚果皮8个菌株分为3种,子叶和胚芽8个菌株电泳图谱共3种,显示了紫穗槐种子内生根瘤菌分布部位选择的偏向性与种类特征的多样性。To determine the amount distribution and category characteristics in seed capsule,pericarp,cotyledon and embryo of rhizobium in Amorpha fruticosa seeds,amount and category of endogenous rhizobium in different parts seed of Amorpha fruticosa were analyzed.The result indicated that there was significant discrepancy in amount distribution and category characteristics in endogenous rhizobium.Most endogenous rhizobia were distributed in seed capsule（6.663×106 cfu/pod）,which accounted for 50 percent of the total strains,and then pericarp（3.335×106 cfu/pod）.Only small amount of endogenous rhizobium was present in cotyledon and embryo（2.087×106 cfu/pod,1.250×106 cfu/pod）.32 strains of rhizobium,isolated from different parts seed of Amorpha fruticosa,were divided into 5 types based on the characteristic maps of RAPDPCR groups（A,B,C,D and E）.Most of the stains were B type,accounting for 40.62% of the total.8 strains belonged to C type,and the number of stains belonging to D,A and E types were 6,4 and 1.There were 5 groups in seed capsule of 16 strains,3 groups in pericarp and only 3 species of cotyledon and embryo.These showed the partiality and diversity of endogenous rhizobium in different parts of seed of Amorpha fruticosa with amount distribution position and category characteristics.

关 键 词：紫穗槐种子 内生根瘤菌 数量分布 种类特征 

分 类 号：Q948.9[生物学—植物学]