系谱和聚类分析在橡胶树内珠被组织培养中应用研究  

The Application of Pedigree and Cluster Analysis in Tissue Culture of Inner Integument in Hevea brasiliensis

作　　者：黄天带[1] 龙青姨[1] 周权男[1] 华玉伟[1] 胡彦师[1] 黄华孙[1] 

机构地区：[1]中国热带农业科学院橡胶研究所农业部橡胶树生物学重点开放实验室省部共建国家重点实验室培育基地海南省热带作物栽培生理学重点实验室,海南儋州571737

出　　处：《热带作物学报》2012年第6期1018-1023,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(30960322);现代农业产业技术体系建设专项(nycytx-34);海南省自然科学基金项目(807043)资助

摘　　要：分析16种不同基因型橡胶树幼果内珠被愈伤组织诱导率、分化率;追踪参试基因型系谱;并基于25对EST-SSRs分子标记数据对其进行聚类分析,研究系谱、聚类结果与体胚发生的关系。结果表明:16种基因型的愈伤诱导率均较高,位于68.46%～100%,7个基因型愈伤组织能诱导出体细胞胚状体,其中3个基因型能够较大量诱导体细胞胚状体,其余9个基因型的愈伤组织完全不具备分化能力;具有RRIM600亲缘的基因型体胚分化能力强,具有IAN873、PB5/51、热研217亲缘的基因型体胚分化能力弱或无体胚分化能力;16种基因型的相似系数位于0.56～0.89间,在遗传距离0.73时分为5类。体胚发生结果与系谱分析结果基本吻合,与聚类分析结果吻合度不大;提示可以利用系谱分析提高橡胶树组织培养的培养基筛选效率。最后讨论分子标记应用于组织培养的研究思路。The young fruits of 16 genotypes were collected to study the callus and embryo induction of inner integument in Hevea. Relationship between tissue culture and pedigree, cluster results were also studied. Results showed that the callus induction frequency was 68.46%-100%; and 7 genotypes were induced somatic embryogenesis with 3 of them induced relatively more embryos while the rest 9 genotypes could not differentiate. The genotypes with RRIM600 phylogenetie relationship showed strong embryogenesis ability, and the genotypes with IAN873, PB5/51 and CATAS217 showed week or no embryogenesis ability. The similarity coefficient was 0.56-0.89, and the 16 genotypes could be divided into 5 categories at 0.73. Somatic embryogenesis ability was highly consistent with pedigree analysis, not with cluster result. It implied pedigree analysis could be used to improve the medium screening efficiency of tissue culture in rubber tree. Finally, the research ideas, of application of molecular markers in tissue culture were discussed.

关 键 词：巴西橡胶树 愈伤诱导 体细胞胚发生 基因型 系谱分析 聚类分析 SSR 

分 类 号：S794.1[农业科学—林木遗传育种]