8个菌株的纤维素分解能力比较研究  

Comparison of Cellulose Decomposing Abilities of Eight Microbial Strains

作　　者：郑鹏[1,2] 李勤奋[2] 李光义[2] 侯宪文[2] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《热带作物学报》2012年第6期1121-1125,共5页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所科研基本业务费项目(2011hzslJ016);现代农业产业体系项目(nycytx-17);公益性行业(农业)科研专项(201203072-04);海南省重点科技项目(ZDXM20100021);海南大学"211工程"建设项目

摘　　要：通过对羧甲基纤维素、滤纸和木薯渣的分解能力、产酶活性等指标比较嗜热侧孢霉、黑曲霉、康宁木霉、白腐菌等8株菌分解纤维素的能力。结果表明:这8株菌均具有较好的纤维素分解能力,其中康宁木霉、白腐菌、无花果曲霉水解圈效果明显;康宁木霉产纤维素酶能力比较稳定,且酶活性较高;康宁木霉、白腐菌、嗜热侧孢霉、无花果曲霉对滤纸和木薯渣的实际分解效果较好。固体发酵培养到第5天时,对粗纤维和纤维素分解率较高的依次为无花果曲霉(23.98%、20.76%)、康宁木霉(23.35%、20.22%)、白腐菌(23.08%、20.16%);固体发酵培养到第10天时,对粗纤维分解率较高的是无花果曲霉(39.22%)、嗜热侧孢霉(36.39%)、米曲霉(35.11%),纤维素分解率较高的是嗜热侧孢霉(42.37%)、无花果曲霉(39.99%)、康宁木霉(38.97%)。To compare the cellulose decomposing abilities of 8 strains, the decomposition capacities of carboxymethyl cellulose, filter paper and cassava residue and their activity were studied. The results showed that： the 8 strains had better cellulose decomposing ability, Trichoderma konigii, Phanerochaete chrysosporium and Aspergillus ficuum had obvious hydrolysis effect; Trichoderma konigii cellulase producing ability was relatively stable and high; Trichoderma konigii, Phanerochaete chrysosporium, Sporotrichum thermophile and Aspergillus ficuum had better decomposition effect on filter paper and the actual cassava residue. In the 5 d, crude fiber and cellulose degradation rate were higher by Aspergillus ficuum （23.98%, 20.76% ）, Trichoderma konigii （23.35%, 20.22% ）and Phanerochaete chrysosporium （23.08%, 20.16% ）and in the 10 d, crude fiber degradation rate was higher by Aspergillus ficuum （39.22%）, Sporotrichum thermophile （36.39%）and AspergiUu oryzae （35.11%）. The cellulose degradation rate was higher by Sporotrichum thermophile （42.37%）, AspergiUus ficuum（39.99% ）and Trichoderma konigii（ 38.97% ）.

关 键 词：纤维素分解菌 酶活性 分解能力 

分 类 号：Q939.96[生物学—微生物学]