缬沙坦对家兔慢性冬眠心肌细胞中肝细胞生长因子水平和心功能的影响  

Effects of valsartan on hepatocyte growth factor and left ventricular function in the rabbit model of chronic hibernating myocardium

作　　者：徐瑞金[1] 纪霞[1] 孙元芬[1] 黎霞[1] 潘茗[1] 

机构地区：[1]青岛市市立医院保健科,266071

出　　处：《中国心血管杂志》2012年第4期294-297,共4页Chinese Journal of Cardiovascular Medicine

摘　　要：目的观察缬沙坦对慢性冬眠心肌细胞中肝细胞生长因子(HGF)和心功能的影响。方法 36只新西兰大白兔用简单随机抽样法分为3组:假手术组、慢性冬眠心肌组、缬沙坦组各12只。结扎冠状动脉左室支制备慢性冬眠心肌模型,在此基础上给予血管紧张素Ⅱ受体(AT1)的拮抗剂缬沙坦干预8周后,用多功能生理记录仪检测左心室功能,酶联免疫吸附法(ELISA)测定心肌组织中HGF含量,末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)检测凋亡细胞。结果与假手术组比较,慢性冬眠心肌组左心室功能减退,心肌组织中HGF含量降低[(2.99±0.57)ng/g组织比(5.16±0.64)ng/g组织,P<0.01];与慢性冬眠心肌组比较,缬沙坦组心肌组织中HGF含量升高[(4.65±0.72)ng/g组织比(2.99±0.57)ng/g组织,P<0.01],细胞凋亡指数降低(99.45±17.32比120.129±15.97,P<0.01),且与心肌组织中HGF含量呈负相关(r=-0.666,P<0.01),缬沙坦可抑制慢性冬眠心肌组织间质纤维化,改善心功能(P<0.01)。结论慢性冬眠心肌中HGF含量降低,缬沙坦可保护缺血心肌。Objective To study the effects of Valsartan on hepatocyte growth factor （HGF） and left ventricular function in the rabbit model of chronic hibernating myocardium. Methods A total of 36 rabbits were randomly divided into three groups （12 in each group） , sham-group, chronic hibernating myoeardium group and Valsartan group. The rabbit model of chronic hibernating myocardium was established by the left ventricle coronary artery ligation. HGF in chronic hibernating myocardium was determined by ELISA and cell apoptosis was detected by TUNEL. Valsartan group was given oral Valsartan for 8 weeks. Left ventricle hemodynamics was monitored by Maclab/12s muhifunctional physiological dynamic instrument. Results Compared with sham-group, left ventricular function was decreased significantly and HGF was significantly lower [ （ 2.99 ± 0. 57 ） ng/g tissue vs. （ 5.16 ± 0. 64 ） ng/g tissue, P 〈 O. 01 ] in chronic hibernating myocardium group. HGF in Valsartan group was significantly higher [ （4. 65±0. 72） ng/g tissue vs. （2. 99 ±0. 57） ng/g tissue, P 〈0. 01 ] than in chronic hibernating myocardium group. The myocardial apoptotic cell in Valsartan group was significantly lower than in chronic hibernating myocardium group （ P 〈 0. 01 ） and was correlated negatively with HGF （ r = - 0. 666, P 〈 0. 01 ）. Conclusions The content of HGF in chronic hibernating myocardium is low. Valsartan could protect ischemic myocardium.

关 键 词：冬眠 心肌 肝细胞生长因子 细胞凋亡 缬沙坦 

分 类 号：R541[医药卫生—心血管疾病]