吸毒人群口腔念珠菌的DNA多态性及其与耐药性的分析  

analysis on DNA polymorphism and the drug resistance of oral candida species from drug abusers

作　　者：袁有华[1,2] 白丽[1] 武有聪[1] 刘奇[1] 钱金栿[3] 郭利军[1] 

机构地区：[1]大理学院基础医学院医学微生物学及免疫学教研室,云南大理671000 [2]河南省南阳市西峡县疾病预防控制中心 [3]大理学院药学院

出　　处：《现代预防医学》2012年第16期4194-4197,4204,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(题目:云南HIV相关性口腔念珠菌病原调查及其生物学研究;C30260005);大理学院博士基金(题目:吸毒成瘾者口腔念珠菌生物学特征及其分布规律的研究;KY430440)

摘　　要：目的了解吸毒人群口腔念珠菌的DNA多态性以及与耐药性的关系。方法选择4种随机引物对75株吸毒人群口腔念珠菌进行DNA多态性分析,并优化试验条件,用聚类分析方法对其进行基因分型。结果筛选出两条随机引物进行RAPD,念珠菌基因组DNA在100～200ng/μL,引物浓度在5～10μmol/L时,得到的PCR指纹图最丰富和清楚。聚类分析显示,吸毒人群口腔念珠菌同种不同株间存在着基因多态性,不同种间具有一定的基因型特点,某些耐唑类和耐5-氟胞嘧啶药物菌株单独聚为特殊的基因型。结论 RAPD基因分型可作为念珠菌种间鉴定的参考方法。吸毒人群口腔念珠菌中某些菌株的耐药性与其DNA多态性有一定关系,某些特殊PCR指纹图可能与耐唑类药物基因和5-FC基因有一定关系。OBJECTIVE To explore the DNA polymorphism and relationship between drug susceptibility and DNA polymor- phism of oral Candida species from drug abusers. METHODS 75 strains of oral eandida were selected, and 4 primers were used for analyzing the DNA polymorphism by RAPD and the experiment condition of RAPD technique was optimized. PCR fin- gerprintings were analyzed for cluster analysis. RESULTS Two pieces of random primer were selected for RAPD-PCR. The fin- gerprintings were more clearer and more abundant when the genome DNA was 100-200ng/p,L and the primer was 5-10p, mol/L. It was shown by cluster analysis that there was genetic polymorphism among different stains of same species and there were some certain genotypic characters among different Candida species from drug abusers＇ oral cavity. Some resistant strains for azoles and 5FC were clustered into special genotype. CONCLUSION RAPD genotype can be as a referenced method to identify different Candida species. There is a likely relationship between drug resistance of some oral Candida strains from drug abusers and DNA polymorphism. Some distinctive PCR fingerprintings of Candida strains are probably relevant to 5-FC and azoles-resistant genes.

关 键 词：念珠菌 随机扩增DNA多态性 吸毒人群 

分 类 号：R379.4[医药卫生—病原生物学]