镍冶炼工人尿镍水平与p15、p16启动子甲基化的关系  

Correlation between Urinary Nickel and Methylation of p15 and p16 in Blood DNA of Workers Exposed to Nickel

作　　者：杨瑾[1] 王志武[1] 郭庆峰[1] 孙建娅[2] 

机构地区：[1]山西医科大学公共卫生学院劳动卫生学教研室,山西太原030001 [2]太原钢铁(集团)有限公司疾病预防控制中心职业病科,山西太原030003

出　　处：《环境与职业医学》2012年第8期485-487,493,共4页Journal of Environmental and Occupational Medicine

基　　金：山西省青年科技研究基金资助项目(编号:2009021044-1);山西省高等学校优秀青年学术带头人支持计划资助(编号:晋教科[2010]4号)

摘　　要：[目的]探讨镍冶炼工人尿镍水平与抑癌基因p15、p16启动子甲基化的关系。[方法]采用丁二酮肟分光光度法测定不锈钢冶炼厂165名镍作业工人(镍接触组)和67名水处理工人(对照组)尿镍浓度,并采用甲基化特异性聚合酶链反应(methylation specific polymerase chain reaction,MSP)检测抑癌基因p15、p16启动子的甲基化状态。[结果]炼钢工、钢渣处理工、轧钢工的尿镍浓度中位数分别为5.34、4.59、4.52μg/L,与水处理工人的尿镍浓度中位数(1.78μg/L)比较,差异均有统计学意义(P<0.01)。镍接触组的p15基因启动子甲基化检出率为25.45%,与对照组甲基化检出率(11.94%)相比,差异有统计学意义(P=0.023);而镍接触组p16基因启动子甲基化检出率(21.82%)与对照组(11.94%)相比,差异无统计学意义(P=0.082)。多元logistic回归分析显示,尿镍浓度高个体的p15甲基化发生可能性为尿镍浓度低个体的1.47倍。[结论]尿镍浓度与p15启动子甲基化的发生有密切关系。[ Objective ] To study the correlation between urinary nickel and methylation of anti-oncogene p15 and p16 in blood DNA of workers exposed to nickel. [ Methods ] Totally 165 nickel exposed workers and 65 water treatment workers without occupational nickel exposure were recruited in this study. The levels of urinary nickel were determined by dimethylglyoxime spectrophotometry. Methylation specific polymerase chain reaction （MSP） was used to detect the methylation of p15 and pl 6 in blood DNA. [ Results ] The median concentrations of urinary nickel in steel refining workers （5.34 p.g/L）, slaggers （4.59 ttg/L） and mill men （4,52 ~tg/L） were significantly higher than that in the controls （1.78 ~tg/L） （P〈 0.01）. The methylation rate ofpl5 in the exposed group （24.55%） was significantly higher than that in the control group （11.94%） （P=0.023）. However, no significant difference in the methylation rate of p16 between the exposed group （21.82%） and the control group （11.94%） was found （P=0.082）. The multivariate logistic analysis showed that workers bearing higher urinary nickel levels were at higher risks of developing p15 methylation （OR=1.47, P=0.024）. [ Conclusion ] The level of urinary nickel is significantly correlated with the methylation of p15 in blood DNA.

关 键 词：镍 抑癌基因 甲基化 

分 类 号：R134.4[医药卫生—劳动卫生]