FGF21对HepG2细胞TGF-β信号通路的影响  

Effect of FGF21 in TGF-β Signaling Pathway of HepG2 Cells

作　　者：江松敏[1] 蔡琳[1] 朱婷婷[1] 施立华[1] 金芩[1] 夏清海[1] 

机构地区：[1]温州医学院药学院,浙江温州325035

出　　处：《中国现代应用药学》2012年第8期675-678,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省教育厅科研项目(Y200907097)

摘　　要：目的研究FGF21对TGF-β/Smads信号通路中相关蛋白和mRNA表达的影响。方法采用Western blot法检测HepG2 cells的TGF-1,TGF-βRⅡ,Smad 2,3,4,7的蛋白表达水平和采用Q-PCR方法检测HepG2 cells的TGF-β1,TGF-βRⅡ,Smad 2,3,4,7的mRNA表达水平。结果在不同FGF21浓度下,TGF-β1,TGF-βRⅡ,Smad 2,3,4,7蛋白和mRNA表达水平不同(P<0.05)。结论 FGF21通过促进TGF-β信号通路中的信号分子或受体的表达,或者通过下调此信号通路的阻断分子而抑制肿瘤发生。OBJECTIVE To study the effects of FGF21 on relative proteins and mRNA expression involved inTGF-β/Smads signaling pathway. METHODS Detect the protein expressions of TGF-β1, TGF-β R Ⅱ, Smad 2,3,4,7 of HepG2 cells with Western blot and detect the mRNA expression of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 of HepG2 cells with Q-PCR. RESULTS The expressions of the protein and mRNA of TGF-β1, TGF-β RⅡ, Smad 2, 3, 4, 7 varied with the different concentrations of FGF21（P〈0.05）. CONCLUSION FGF21 might inhibit tumor by promoting TGF-β signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking molecules of this signaling pathway.

关 键 词：FGF21 HEPG2细胞 SMAD 

分 类 号：R965.1[医药卫生—药理学]