HPLC测定翅茎白粉藤中白黎芦醇的含量  

Determination of Resveratrol in Cissus Hexangularis by HPLC

作　　者：刘元[1] 宋志钊[1] 文志云[1] 

机构地区：[1]广西中医药研究院,南宁530022

出　　处：《中国现代应用药学》2012年第8期734-736,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：广西科技攻关项目(桂科攻0992003A-22);广西壮药质量标准(第二卷)标准研究项目(MZY2010054);广西壮族自治区卫生厅自筹经费科研课题(Z2011276)

摘　　要：目的建立翅茎白粉藤中白黎芦醇的含量测定方法。方法采用高效液相色谱法,色谱柱XB-C18（4.6 mm×250 mm,5μm）,以乙腈-水（25∶75）为流动相,检测波长为306 nm,柱温为室温。结果白黎芦醇在2.50~40.0μg内峰面积与其浓度呈良好线性关系（r=0.999 9）,平均回收率为97.3%,RSD为0.98%（n=6）。结论本方法简便、准确、重复性好,可作为该药材含量测定方法。OBJECTIVE To develop an HPLC method for detecting resveratrol in Cissus hexangularis. METHODS An XB-CIg column（4.6 mm×250 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-water（25 ： 75）. The detection wavelength was 306 nm and the column temperature was room temperature. RESULTS Resveratrol showed a good linear relationship in the range of 2.50-40.0 μg.mL^-1, r=0.999 9. The recovery and RSD were 97.5% and 0.98%（n=6）, respectively. CONCLUSION This method is simple, accurate and repeatable to determine the content ofresveratrol in Cissus hexangularis.

关 键 词：翅茎白粉藤 高效液相色谱法 白黎芦醇 

分 类 号：R917.101[医药卫生—药物分析学]