乙型肝炎病毒e抗原对人外周血淋巴细胞的影响  

Effect of HBe-antigen on Human Peripheral Blood Lymphocytes

作　　者：蒋永芳[1] 唐伟[1] 马静[1] 贺波[1] 李耐萍[1] 龚国忠[1] 

机构地区：[1]中南大学湘雅二医院肝病研究中心,湖南长沙410011

出　　处：《医学临床研究》2012年第7期1264-1266,共3页Journal of Clinical Research

摘　　要：【目的】探索乙型肝炎病毒（HBV）感染后，乙型肝炎e抗原（HBeAg）在免疫调节中的作用和机制。【方法】分离正常人血淋巴细胞，分别加入不同浓度的HBeAg，培养72h后，计数细胞，流式细胞仪分析T淋巴细胞亚群的改变，同时分析细胞Toll受体（TLR）中TLR3、TLR4和细胞程序性死亡受体-1（PD-1）的表达率，ELISA分析7干扰素（IFN-γ）浓度。【结果】淋巴细胞的增殖随HBeAg浓度增加而明显抑制，CD4＋和CD8＋细胞百分比也相应降低，TLR3、TLR4和PD-1的表达率明显增高，人γ干扰素（IFN-γ）浓度降低。【结论】HBeAg体外能抑制淋巴细胞生长，使T淋巴细胞比例降低，促进PD-11的表达并抑制其活性。[Objective] To explore the role of HBeAg in the immunological regulation of HBeAg after hep- atitis B virus（HBV） infection and its mechanism. [Methods] HBeAg with different concentration were added into lymphocytes isolated from blood of normal people. After cultivation for 72h, cell count and flow cytome- try were used for analyzing the change of T-lymphocyte subsets. Meanwhile the expression of Toll-like recep- tors（TLR） such as TLR3 and TLR4 and programmed cell death receptor-1 （PIll） were analyzed. ELISA was used to analyze IFN-γconcentration. [Results] With the increasing of HBeAg concentration, the proliferation of lymphocytes was inhibited, as well as the percentage of CD4＋ and CD8＋ lymphocytes correspondingly de- creased, but the expression of TLR3, TLR4 and PD-1 were increased while the expression of INF-γ decreased. [Conclusion] HBeAg can inhibit the proliferation of lymphocytes, decrease the percentage of T cells, promote the expression of PD-1 and inhibit its activity in vitro.

关 键 词：肝炎E抗原 乙型 淋巴细胞 

分 类 号：R512.62[医药卫生—内科学]