转化生长因子βⅡ型受体在病理性瘢痕表皮组织中的基因表达研究  

Gene expression of transforming growth factor βreceptor Ⅱ in the epidermis of pathological scar

作　　者：陈铭锐[1] 安纲[2] 刘顺利[1] 魏奉才 

机构地区：[1]济南军区总医院烧伤整复科,250031 [2]山东省千佛山医院整形外科 [3]山东齐鲁医院口腔外科

出　　处：《中华烧伤杂志》2012年第4期278-281,共4页Chinese Journal of Burns

摘　　要：目的了解病理性瘢痕表皮组织中TGF-βⅡ型受体（TβRⅡ）的基因表达。方法2（）份病理性瘢痕标本收集于2007-2009年济南军区总医院收治的20例烧伤或外伤瘢痕患者，于每份瘢痕标本中央和边缘部位分别切取1块表皮组织。每例患者留取1块距离病变部位10cm以上的正常皮肤组织标本作为自身对照1。抽取每例患者1～2mL全咀并分离提取血清，作为自身对照2。另取8例无病理性瘢痕病史患者术中弃用的8份正常皮肤组织标本作为正常对照。生物素．链霉亲和索-过氧化物酶染色法检测3种组织标本中TβRⅡ的阳性表达。经PCR-单链构象多态性分析、基㈧洲序，对比观察各标本中TβRⅡ的基因表达。对数据行Fishm。确切概率法检验。结果免疫组织化学染色结果显示，病理性瘢痕表皮组织标本中TβRⅡ阳性表达明鼎低于自身对照1和正常对照组织标本，主要位于表皮的基底层，而棘层、颗粒层和角质层中TβRⅡ阳性表达很少或缺失。在自身对照1、自身对照2和正常对照标本中未见TβRⅡ基因异常表达；在8份病理性瘢痕表皮组织标本中TβRⅡ多聚A位点的片段出现条带泳动异常，基闪测序娃示DNA片段缺失1个A（P=0．044）。结论病理性壤痕表皮组织中可能存在TβRⅡ基因的异常表达．．瘢痕表皮回植可能会增加病理性搬痕复发的风险，而切取瘢痕患者正常皮肤植皮修复创呵可能不会增加瘢痕复发的风险。Objective To study the gene expression of transforming growth factor β receptor Ⅱ （TβRⅡ ） in pathological scar. Methods Twenty samples of pathological scar were collected from 20 burn or trauma patients hospitalized in the General Hospital of Ji＇nan Military Command from 2007 to 2009. Twenty specimens of epidermal layer were obtained from the middle portion and the edge of pathological scars. Twenty normal skin specimens which were located more than 10 cm away from the lesion sites of 20 patients were collected as self-controls. Serum from 1-2 mL whole blood were obtained from each of the 20 patients for second self-contrnl. Eight normal skin specimens from 8 patients without pathological scar, discarded from un-related operations, were also collected as negative,-control. Positive expressions of TβRⅡ in three different skin specimens were determined with biotin-streptavidin-peroxidase staining. Gene expressions of TβRⅡ in all specimens were compared with PCR-single strand conformation polymorphism analysis and gent sequencing. Data were processed with Fisher＇s exact test. Results Positive expression of TβRⅡin pathological scar epidermis was lower than that in normal skin specimen of patients with pathoh;gical scar or normal skin specimen of patients without pathological scar, and TβRⅡ was mainly located in the basal layer of epidermis. Positive expressions of TβRⅡ were seldom found in acanthocytes, granular cells, and cuticle or even non-existing. No abnormality of TβRⅡwas fnund in nnrmal skin epidermis or serum samples of pathological scar patients or normal skin epidermis of palients without pathological scar.TβRⅡ expressing in 8 specimens of epidermis of pathological scar showed abnormal electrophoresis pattern at poly A fragments hand and loss of one Ahase in DNA fragment （ P = 0. 044）. Conclusions There may be abnormal gene expression of TβRⅡ in pathological scar epidermis. Replantation of epidermis of scar may increase the risk of scar recurrence, while replantat

关 键 词：受体 转化生长因子Β 表皮 基因表达 病理性瘢痕 

分 类 号：R622[医药卫生—整形外科]