锌对甲基汞染毒大鼠氧化应激损伤的抑制作用  

Inhibitive Effects of Zinc on Methylmercury-induced Oxidative Reactive Damage in Rats

作　　者：石勇[1] 石琦[2] 宋祥福[1] 刘航[3] 阎兆宏[4] 

机构地区：[1]吉林大学公共卫生学院卫生检验教研室,长春130021 [2]吉林大学白求恩医学院,长春130021 [3]吉林大学药学院,长春130021 [4]吉林大学第一医院,长春130021

出　　处：《实验动物与比较医学》2012年第4期304-307,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的探讨锌对甲基汞急性染毒大鼠组织及血液各氧化指标的变化。方法大鼠随机分为锌＋甲基汞组、甲基汞组和对照组，锌＋甲基汞组大鼠每目腹腔注射不同剂量硫酸锌，剂量分别为2．86、5．72、8．58、11．44mg／kg，第6日甲基汞急性染毒，第7日处死，取大鼠肝、脑、肾及血液分别测定谷胱甘肽过氧化物酶（GSH-Px）活性、超氧化物歧化酶（SOD）活性。结果随着锌剂量的增加，大鼠组织和血液中GSH—Px活性降低，GSH—Px活性与锌剂量呈显著负相关；随着锌剂量的增加，大鼠组织和血液中SOD活性增强，锌＋MeHg实验组中SOD活性与锌剂量呈显著正相关关系。结论锌能有效预防甲基汞染毒大鼠的机体损伤。Objective To investigate the changes of tissue and hematological oxidation in acute methyl mercury-contaminated rat. Method The total of rats were randomly divided into three groups, zinc＋methyl mercury groups, methyl mercury exposure group and control group, zinc＋methyl mercury groups were treated with different doses of ZnSO4 （2.86, 5.72, 8.58, 11.44 mg/kg） by intraperitoneal injection every day. On the 6th day, the rats in all groups except control group were exposed to methylmecury by oral administration. All the rats were sacrificed on the 7 th day, the activity of super- oxide dismutase （SOD） and glutathione peroxidase （GHS-Px） in the liver, brain, kidney and blood were determined. Results With the zinc＋methyl mercury groups, compare to the treatment of methylmecury, the activities of SOD significantly increased （P〈0.05） and GSH-Px significantly decreased （P〈0.05） in the tissues and blood, and shown a significant positive correlation. Conclusion The Zinc has signifi- cant inhibitive effect on methylmercury-induced oxidative damage in rats.

关 键 词：锌 甲基汞 超氧化物歧化酶 谷胱甘肽过氧化物酶 金属硫蛋白 

分 类 号：R99[医药卫生—毒理学] R-332[医药卫生—药学]