高效液相色谱法测定左归丸中马钱苷的含量  

Determination of Loganin in Zuogui Pill by High Performance Liquid Chromatography

作　　者：孙琳林[1] 陈海玲[2] 卢林[1] 刘伦翠[1] 刘艳秋[1] 

机构地区：[1]牡丹江医学院红旗医院中西医结合科,黑龙江牡丹江157011 [2]杭州市中医院老年病科,浙江杭州310007

出　　处：《中华中医药学刊》2012年第8期1792-1793,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：黑龙江省中医药管理局支撑项目(ZHY10-W70);黑龙江省卫生厅科技计划支撑项目(2010-281);杭州市科技发展计划项目(20100633B22)

摘　　要：目的:建立测定左归丸中马钱苷含量的反相高效液相色谱方法。方法:采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);以四氢呋喃-甲醇-乙腈-水(1∶4∶8∶87)为流动相;流速为1.0mL.min-1;柱温为40℃;检测波长236nm。结果:马钱苷进样浓度在0.0079～0.2530mg.mL-1(r=0.9993)范围内呈良好的线性关系,平均回收率(n=6)为101.6%;RSD为2.1%。结论:该方法简便、重现性好,结果准确可靠,可以用于左归丸中马钱苷的含量测定,为左归丸的质量控制提供一定的理论依据。Objective：To develop a method for determination of loganin in Zuogui pill by high performance liquid chromatography（HPLC）.Methods：A HPLC system was used in the quantitative analysis of hesperidin on the reversed-phase column of Ec1ipse XDB-C18（4.6mm×250mm,5μm）with the mixed solvents of tetrahydrofuran-methanol-acetonitrile-0.05% phksphoric acid water solution.The flow rate was 1.0mL/min.Column temperature was 40℃.The eluate was monitored with UV absorption at 236nm.Results：The linear range of hesperidin was 0.0079 ~ 0.2530mg/mL（r =0.9993）.The average recoveries（n=6）were 101.6%,with RSD of 2.1 %.Conclusion：The method is simple,accurate and reliable with good reproducibility.It can be used to evaluate and control the quality of Zuogui pill.

关 键 词：左归丸 马钱苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]