羊栖菜多糖对老年痴呆模型大鼠Bcl-2和Bax基因表达的分析  

Effect of Sargassum fusiforme Polysaccharide on the Ethology and Expressions of Bcl-2 and Bax in Brain Tissue for Alzheimer's Disease Rat model

作　　者：汤从容[1] 曹高忠[1] 叶晓兰[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325035

出　　处：《中华中医药学刊》2012年第8期1832-1834,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：温州市科技局资助项目(Y20090429)

摘　　要：目的:探讨羊栖菜多糖提取物(SFPS)对阿尔茨海默病(AD)大鼠模型行为的干预作用及脑皮质Bcl-2和Bax基因表达的影响。方法:制作D-半乳糖阿尔茨海默病大鼠模型,设计正常对照组、模型组、吡拉西坦片、羊栖菜多糖提取物不同剂量组,观察大鼠行为学及脑皮质Bcl-2和Bax基因表达指标的改变。结果:与正常对照组相比,模型组学习记忆能力显著下降(P<0.05),其Bcl-2/Bax值下降;与模型组相比,0.8g/kg、1.6g/kg羊栖菜多糖提取物治疗组均能较好的改善学习记忆能力,且具有一定剂量依赖性,其Bcl-2/Bax值增加。结论:SFPS能调节海马组织Bcl-2和Bax的表达,显著提高Bcl-2/Bax值,抑制海马神经元的凋亡,改善AD大鼠学习记忆能力。Objective：To study effect of SargassumfusiJbrme Polysaceharide（SFPS） on ethology and expressions of Bel - 2 and Bax in brain tissue of AD rats. Methods ： The D - galactose AD rat model was applied, and blank group, model group, Piracetam control group and herbal group were designed, index change of ethology, expressions of Bcl -2 and Bax in brain tissue were tested. Results：AD model may decrease cognitive ability,the ratio of Bcl -2/Bax in the hippoeampus came down （ P 〈 0.05 ）. Compared to the model group, Sargussum fusiforme Polysaccharide with O. 8,1.6g/kg can allevi- ate cognitive ability significantly, and the action had a does -dependent increase, the ratio of Bcl -2/Bax increased（ P 〈 0.05 ）. Conclusion ： Sargassum fusiforme Polysaccharide can up - regulate expression of protein Bax and down - regulate expression of Bcl -2 in brain tissu. It inhibits apoptosis through regulating the expressions of Bcl -2 and Bax protein in hippocampal, which might be one of mechanisms of Sargassum fusiforme Polysaccharide to prevent and treat AD disease.

关 键 词：阿尔茨海默病 羊栖菜多糖提取物 学习记忆 脑皮质Bcl-2和Bax基因表达 

分 类 号：R285.5[医药卫生—中药学]