三七总皂苷对以尿酸钠诱导血管内皮细胞凋亡的影响  

Effect of Panax Notoginsenosides on Vascular Endothelial Cells Apoptosis Induced by Sodium Urate(MSU)

作　　者：张福康[1] 杨妍华[1] 陆再华 

机构地区：[1]常州市第七人民医院,江苏常州213011

出　　处：《中华中医药学刊》2012年第8期1843-1845,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：常州市科技计划项目(CS20109011)

摘　　要：目的:观察三七总皂苷片剂对以尿酸钠(MSU)100μg.mL-1诱导血管内皮细胞凋亡的影响。方法:体外培养的血管内皮细胞,以尿酸钠(MSU)100μg.mL-1为凋亡诱导因子,建立血管内皮细胞凋亡诱导模型,继而采用2.5、5、10μg.mL-1浓度的三七总皂苷进行干预,用流式细胞仪测定细胞凋亡率,采用实时定量PCR检测ICAM-1基因的表达。结果:三七总皂苷组2.5μg.mL-1明显抑制细胞凋亡(6.9±1.07)%,与模型组比较P<0.01;能显著抑制ICAM-1基因的表达。结论:三七总皂苷能明显抑制以尿酸钠(MSU)100μg.mL-1诱导的血管内皮细胞凋亡。Objective：To evaluate the effect of Panax Notoginsenosides on MSU-induced apoptosis of vascular endothelial cells.Methods：The induced-apoptosis model built in vitro was cultured vascular endothelial cells with MSU（100μg·mL-1）as the apoptosis factor and triggered by 2.5μg·mL-1 of Panax Notoginsenosides in advance.The apoptosis ratio was monitored by real-time Q-PCR to measure the expression of ICAM-1 gene on a flow-cytometer.Results：Comparing with the model group,Xuesaitong（2、5、10μg·mL-1）drastically inhibited the cell apoptosis[（6.9±1.07）%] by notably suppressing the expression of ICAM-1 gene.Conclusions：The Panax Notoginsenosides can drastically inhibit the apoptosis of vascular endothelial cells induced by MSU（100μg·mL-1）.

关 键 词：三七总皂苷 细胞凋亡 痛风 

分 类 号：R285.5[医药卫生—中药学]