CXCL9对单个核细胞的趋化作用及其机制探讨  

Chemotaxis of CXCL9 to human peripheral blood mononuclear cells and its mechanism

作　　者：夏羽佳[1] 田德安[1] 曾丹[1] 何嘉怡[1] 涂炜[1] 邓欢[1] 韩平[1] 邓玥灵[1] 刘梅[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2012年第4期319-322,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(30972759;810703-33)

摘　　要：目的观察趋化因子CXCL9对人外周血单个核细胞的趋化作用,并探讨其对CXCR3受体后信号通路的影响。方法分离人外周血单个核细胞并进行培养,Transwell小室趋化实验检测不同浓度的趋化因子CXCL9对外周血单个核细胞的趋化作用;Western blot方法检测CXCL9刺激外周血单个核细胞时ERK1/2及PI3K/Akt信号通路的蛋白表达变化,并检测上述通路抑制剂PD98059和Wortmannin处理细胞后,CXCL9对ERK1/2、PI3K/Akt信号通路的影响有无变化。结果与空白对照组相比,不同浓度的CXCL9刺激对人外周血单个核细胞均有明显的趋化作用,并且CXCL9刺激人外周血单个核细胞能激活ERK1/2及PI3K/Akt信号通路,其关键蛋白ERK1/2及Akt磷酸化水平显著增加;通路特异性抑制剂PD98059和Wortmannin的应用能明显抑制CXCL9对这两条信号通路的激活。结论 CXCL9能趋化人外周血单个核细胞发生迁移,ERK1/2及PI3K/Akt信号通路可能在此过程中发挥重要作用。Objective To investigate the chemotaxis of CXCL9 to human peripheral blood mononucle- ar cells （PBMCs） and explore its signaling pathway. Methods PBMCs were isolated and cultured. The chemotaxis of CXCL9 to PBMCs was measured by Transwell assay. With CXCL9 treatment, the expression of ERK1/2 and Akt protein were detected in PBMCs by Western blot analysis. After inhibitor PD98059 or Wortmannin treatment, the changes Compared with the control, migration of PBMCs of ERK1/2 and Akt protein were determined. Results was observed in response to CXCL9 treatment after the 4h time frame of the experiment. In addition, the ERK1/2 and PI3K/Akt signaling pathways were activa- ted and the phosphorylated ERK1/2 and phosphorylated Akt were substantially increased. The ERK1/2 and PI3K/Akt inhibitors （PD98059 and Wortmannin） suppressed the activation of the two signaling path- ways induced by CXCL9. Conclusion CXCL9 induces signaling pathways may play an important role in this the migration of PBMCs, and ERK1/2, PI3K/Akt process.

关 键 词：趋化因子 CXCL9 单个核细胞 趋化作用 信号通路 

分 类 号：R349.54[医药卫生—基础医学]