p120ctn、Kaiso及matrilysin在非小细胞肺癌中的表达及其意义  

Expression and significance of p120ctn,Kaiso and matrilysin in non-small cell lung cancer

作　　者：梁键[1,2] 秦生辉[1,2] 张艳丽[1,2] 李娜萍[1,2] 吴人亮[1,2] 王曦[1,2] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所 [2]华中科技大学同济医学院病理学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2012年第4期340-345,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(81070009)

摘　　要：目的探讨p120catenin(p120ctn)、Kaiso和matrilysin在非小细胞肺癌(non-small cell lung cancer,NSCLC)发生发展中的作用。方法采用免疫组织化学法检测98例NSCLC(52例肺鳞状细胞癌和46例肺腺癌)以及16例支气管扩张症组织中p120ctn、Kaiso和matrilysin的表达情况,分析NSCLC中p120ctn、Kaiso和matrilysin与临床病理特征的关系及三者表达的相关性。结果在NSCLC组织和支气管扩张症组织中,p120ctn蛋白异常表达率分别为74.5%、6.3%,两组之间表达率比较有统计学差异(P<0.001);在肺癌细胞、杯状细胞、纤毛细胞、基底细胞中Kaiso蛋白异位表达率分别为63.3%、6.3%、6.3%、37.5%,肺癌细胞与杯状细胞、纤毛细胞表达率比较有统计学差异(P<0.001)。p120ctn蛋白异常表达、matrilysin蛋白细胞质表达均与NSCLC组织学类型密切相关(P<0.05),Kaiso蛋白异位表达与NSCLC分化程度密切相关(P<0.05)。在NSCLC组织中,Kaiso细胞核表达与matrilysin细胞质表达存在显著负相关(r=-0.23,P=0.02)。结论 p120ctn可能通过与Kaiso在胞质内异位表达,解除了Kaiso对靶基因的抑制作用,促进matrilysin的细胞质表达,而参与肺癌的发生发展。Objective To investigate the expression of pl20ctn, Kaiso and matrilysin proteins in non- small cell lung cancer （NSCLC） tissues, and to analyze their effect on tumorigenesis and development. Methods The expression of pl20ctn, Kaiso and matrilysin proteins were detected using immunohistoehem- istry in 16 cases of bronchiectasis and 98 cases of NSCLC, including 52 cases of lung squamous cell carci- noma and 46 cases of lung adenocarcinoma. The relationship between p120ctn, Kaiso, matrilysin and clini- copathologic characteristics was analyzed. Results The abnormal expression rates of pl20ctn were 74.5 %, 6.3% in NSCLC and bronchiectasis specimens respectively, and the difference showed statistical signifi- cance （P^0. 001）. The ectopic expression rates of Kaiso in lung cancer cells, goblet cells, ciliated cells and basal cells were 63.3%, 6.3%, 6.3%, 37.5%, respectively, and the difference of ectopic expression rates between lung cancer cells and goblet cells or ciliated cells was statistically significant（ P〈0. 001）. The abnormal expression of p120ctn and cytoplasmic matrilysin expression in patients with NSCLC were both related to histological type （P〈0.05）. The ectopic expression of Kaiso in patients with NSCLC was related to the grade of differentiation （P〈0.05）. Nuclear Kaiso expression was correlated with cytoplas- mic matrilysin expression （r=-0.23, P=0.02）. Conclusion P120 may play a role in the NSCLC develop- ment through co-localization with Kaiso in the cytoplasm, relieving the repression of Kaiso to its target gene, and promoting matrilysin expression.

关 键 词：非小细胞肺癌 P120连环蛋白 Kaiso 基质溶素 

分 类 号：R322.24[医药卫生—人体解剖和组织胚胎学]