HPLC法测定防芷鼻炎片中芍药苷的含量  

Content Determination of paeoniflorin in Fangzhibiyan Tablets by HPLC

作　　者：梁可文 龙云 施丹 

机构地区：[1]广西健丰药业有限公司,广西南宁530104

出　　处：《中国民族民间医药》2012年第16期67-68,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立防芷鼻炎片中芍药苷含量测定的方法。方法:采用高效液相色谱法。色谱柱为AICHROM(C18,4.6mm×250mm,5μm),流动相为乙腈-0.05mol.L-1磷酸二氢钾溶液(12:88),流速为1.0 mL.min-1,检测波长为230nm,进样量为10μl,柱温为室温。结果:芍药苷检测浓度在10.4～52.0μg.ml-1范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为99.41%,RSD=1.55%(n=6)。结论:本方法简便、易行、重复性好,可作为防芷鼻炎片中芍药苷含量的测定方法。OBJECTIVE： To establish a method for the content determination of paeoniflorin in Fangzhibiyan Tablets. METH- ODS： HPLC methof was adopted. The AICHROM （C18, 4. 6 × 200mm, 5μm） was used and mobile phase was acetonitrile - 0. 05mol · L- 1 dihydrogen phosphate armour solution （12：88 ） , and its temperature was used at room temperature, with the flow rate of 1.0 ml · min^- 1 and the wavelength of detection of 230 nm, and the injection volume was 10pd. RESULTS： The linear range of pro- caine was 10. 4 -52. 0μg · ml^-1 （r =0. 9998） with an average recovery of 99.41% （RSD = 1.55% ）. CONCLUSION： The meth- od is simple, practical and well reproducible. It can be used as a method for the content dedermination of Fangzhibiyan Tablets.

关 键 词：防芷鼻炎片 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]