游离脂肪酸对滋养细胞线粒体长链脂肪酸氧化酶表达的影响  

Effects of expression of mitochondria long-chain fatty acid oxidative enzyme with different chain lengths of free fatty acids in trophoblast cells

作　　者：孙晓乐[1] 杨孜[1] 王晓晔[1] 王伽略[1] 武淑英[1] 

机构地区：[1]北京大学第三医院妇产科,100191

出　　处：《中华医学杂志》2012年第29期2034-2037,共4页National Medical Journal of China

基　　金：基金项目：国家自然科学基金（30973204）;北京市自然科学基金（7102162）

摘　　要：目的探讨不同链长游离脂肪酸对人滋养细胞线粒体脂肪酸β氧化循环中长链三羟基酰基辅酶A脱氢酶（LCHAD）基因和蛋白表达变化的影响。方法体外无血清培养人绒毛滋养细胞，将细胞分为五组，每组用不同链长脂肪酸孵育细胞，分别为无游离脂肪酸（F-FFA）、短链脂肪酸（SC—FFA）、中链脂肪酸（MC—FFA）、长链脂肪酸（LC—FFA）、极长链脂肪酸（VLC-FFA）。采用实时荧光定量PCR和蛋白印迹法检测各组LCHAD基因和蛋白的表达变化。结果与F-FFA、SC—FFA、MC—FFA3组比较，LC—FFA组LCHAD基因和蛋白明显降低（P〈0．05），VLC—FFA组LCHAD基因明显升高（P〈0．05），而蛋白表达差异无统计学意义（P〉0．05）。F-FFA、SC—FFA、MC—FFA3组之间LCHAD基因和蛋白表达差异无统计学意义（P〉0．05）。与LC—FFA组比较，VLC-FFA组LCHAD基因和蛋白明显升高（P〈0．05）。结论游离脂肪酸对人滋养细胞线粒体脂肪酸B氧化循环LCHAD的基因蛋白表达变化存在影响；LC—FFA刺激下的胎盘滋养细胞中存在LC-FFA线粒体β-氧化代谢障碍。VLC-FFA与脂肪酸β-氧化代谢存在一定的相关性，其相互作用机制还有待进一步研究。短链、中链脂肪酸未显示对线粒体脂肪酸β-氧化代谢的影响。Objective To explore the interacting mechanisms and influences of different chain lengths of fatty acids and the expression of mitochondria long-chain 3 hydroxyacyl CoA dehydrogenase （LCtlAD） in trophoblast cells. Methods The serum-free trophoblast cells cultured in vitro were divided into S groups to receieve the stimulations of DMEM/F12 medium without FFA （F-FFA）, short-chain fatty acids （SC-FFA）, medium-chain fatty acids （MC-FFA）, long-chain fatty acids （LC-FFA）, very long-chain fatty acids （VLC-FFA）. The expressions of mRNA and protein of LCHAD in trophoblast cells were detected by real-time polymerase chain reaction （PCR） and Western blot. Results Compared with the F-FFA, SC- FFA and MC-FFA groups, the expressions of gene and protein of LCHAD significantly decreased （P 〈 0. 05） in the LC-FFA group. The expression of gene of LCHAD increased significantly in the VLC-FFA group （ P 〈 0.05 ）. But no difference existed in protein expression between the VLC-FFA group and other three groups （P 〉 0. 05）. Gene expression of LCHAD had no difference among the F-FFA, SC-FFA, MC- FFA groups （P 〉 0.05 ）. Compared with the LC-FFA group, the expression of gene of LCHAD increased significantly in the VLC-FFA group （P 〈 0. 05 ）. Conclusion Free fatty acids may affect the expression of mitoehondrial β-oxidation enzyme of LCHAD in trophoblast ceils. Long-chain fatty acid alters the LCHAD gene protein expression. The correlation between very long chain fatty acids and the gene expression of LCHAD has been detected and their interactions needs further explorations. Short or medium chain fatty acids have no significant effect on the mitochondrial metabolism of fatty acid β-oxidation in trophoblast cells.

关 键 词：游离脂肪酸 LCHAD 脂肪酸氧化 滋养细胞 

分 类 号：R714.245[医药卫生—妇产科学]