川芎嗪对废用状态下大鼠骨骼肌一氧化氮合酶活性及肌纤维内Ca2＋浓度的影响  

Effects of tetramethylpyrazine on nitric oxide synthase activity and calcium ion concentration of skeletal muscle in hindlimb unloading rats

作　　者：武雪[1] 高云芳[1] 赵雪红[2] 崔继红[1] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]襄樊学院医学院医学机能学部

出　　处：《中华医学杂志》2012年第29期2075-2077,共3页National Medical Journal of China

基　　金：基金项目：国家自然科学基金（30770273）;2011年度高等学校博士学科点专项科研基金（20116101110013）;陕西省教育厅省级重点实验室重点科研计划（11JS092）

摘　　要：目的通过研究川芎嗪对废用状态下大鼠骨骼肌一氧化氮合酶（NOS）活性及肌纤维内钙离子浓度的影响，探讨川芎嗪对抗废用性肌萎缩的可能机制。方法采用尾部悬吊法建立大鼠后肢废用模型，比色法测定NOS的活性；激光扫描共聚焦显微镜技术测量骨骼肌单纤维的钙荧光强度。18只雌性SD大鼠分为3组，正常对照组（CON）、吊尾＋溶媒灌胃组（HLU＋W）、吊尾＋川芎嗪最佳剂量组（HLU＋Tmp）。结果（1）HLU＋w组NOS活性比CON组低28％（P〈0．05），HLU＋Tmp组比CON组低46％（P〈0．01）。HLU＋Tmp组的NOS活性低于HLU＋w组，但差异无统计学意义（P〉0．05）。（2）HLU＋w组和HLU＋Trap组的骨骼肌肌纤维钙荧光强度分别比CON组高330％与86％（P〈0．01）。HLU＋Tmp组比HLU＋w组低130％（P〈0．01）。结论废用状态下，骨骼肌肌纤维的NOS活性降低，钙离子浓度升高。川芎嗪可抑制骨骼肌废用引起的胞质钙超载，但不能抑制废用引起的NOS活性降低。Objective To explore the effects of tetramethylpyrazine on the nitric oxide synthase activity and calcium ion concentration in skeletal muscle fiber and decipher the possible mechanisms of antimuscle atrophy function of tetramethylpyrazine in hindfimb unloading rats. Methods Hindlimb unloading （HLU） rats were used as a muscle atrophy model to study the activity of nitric oxide synthase by colorimetry. The concentration of intracellular calcium ion was measured by laser scanning eonfocal microscope. A total of 18 female rats were randomly divided into 3 groups： control （CON）, hindlimb unloading with water （HLU ＋ W） and hindlimb unloading with tetramethylpyrazine （HLU ＋ Trap） （n = 6 each）. Results （ 1 ） Compared with CON, the activity of nitric oxide synthase decreased by 28% in HLU ＋ W（P 〈 0.05 ）and decreased by 46% in HLU ＋ Trap（ P 〈 0.01 ）. The activity of nitric oxide synthase less decreased in HLU ＋ Tmp than that in HLU ＋ W, but it was not statistically significant （ P 〉 0.05 ）. （ 2 ） Compared with CON, the concentrations of intracellular calcium ion in HLU ＋ W and HLU ＋ Trap increased by 330% and 86% respectively （ P 〈 0.01 ）. Compared with HLU ＋ W, the concentration of intracellular calcium ion decreased by 130% in HLU ＋ Trap （ P 〈 0.01 ）. Conclusion The activity of nitric oxide synthase decreases and the concentration of calcium ion increases in hindlimb unloading rats. And tetramethylpyrazine may suppress the calcium ion overloading but not the activity of NOS associated with disuse muscular atrophy.

关 键 词：废用性肌萎缩 一氧化氮合酶 钙离子 川芎嗪 

分 类 号：R285.5[医药卫生—中药学]